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Psyche 
[Vol. 90 
Results of Dissecting Symmyrmica chamberlini 
The three samples were kept alive for several months. However, 
numerous specimens died during the first few weeks. A number of 
them could be dissected following the method described by Busch- 
inger and Alloway (1978). 
In a total of 15 ordinary workers without any vestiges of ocelli on 
their heads, the number of ovarioles was always two, except in one 
specimen which has three. No spermatheca could be found in any of 
these workers. 
On the contrary, we found five slightly intermorphic specimens, 
with between one and three more or less perceptible ocelli, with 
somewhat deeper thoracic sutures, and with 6 ovarioles and a 
spermatheca each. Two of these specimens, both from colony no. 2 
(where males had been present), contained living sperm in their 
receptacles. Their ovarioles, however, were short and transparent as 
is usual in young, not yet egg-laying females. 
Additional observations were made referring to the abdominal 
glands of S. chamberlini. Thus, the poison gland reservoir was 
always of usual size and shape, as in other leptothoracine ants. The 
Dufour’s gland, however, is large both in workers and intermorphic 
females. Its size exceeds considerably that of independent Lepto- 
thorax species, and it reaches that of, e.g., Harpagoxenus sublaevis 
(Buschinger and Alloway 1978). 
A karyological study of 7 prepupae from colony no. 2 was made 
following the method of Imai et al. (1977). The results, however, 
were not as good as to permit the presentation of a karyotype. We 
could only determine the chromosome number, which is 2n = 28. 
Laboratory Observations 
We were not able to take large samples of the Manica host species 
with us alive. So only very few observations of interactions between 
chamberlini and their hosts were possible. However, following a 
method which had already worked with Formicoxenus nitidulus 
(Buschinger 1976), we tried to join chamberlini brood and adults 
with an unnatural host species. We chose a Leptothorax species 
which was nesting within dead willow stems near to our chamberlini 
site. Apparently it represents an unknown, new species belonging to 
the subgenus Leptothorax (= Mychothorax Ruzsky). The following 
experiments and observations were made: 
