1981] Howard, Mallet te, Haver tv, & S my the — Reticulitermes 77 
Also during this period dead dealates were replaced with live ones. 
After the first week dead dealates were removed but not replaced. 
Subsequent inspections were made approximately three times a 
week for the following month, then two times a week for 3 months, 
once a week for 2 months, and once every 2 weeks thereafter until 
the dealates were dead or the experiment was terminated. At each 
inspection the number of live dealates, eggs, larvae (by stage), 
presoldiers, and soldiers were recorded. 
Seven combinations involving the following pairings were exam- 
ined: (1) R. flavipes male + R. flavipies female (spring); (2) R. 
flavipes male + R. flavipes female (fall); (3) R. virginicus male + 
R. virginicus female; (4) R. flavipes male + R. virginicus female; (5) 
R. virginicus male + R. flavipes female; (6) R. flavipes female + R. 
flavipes female; and (7) R. virginicus female + R. virginicus female. 
Each combination was replicated a minimum of ten times per year. 
Determination of Larval Stage 
Larvae from six of the seven combinations were randomly 
selected from at least five of the experimental units in each 
combination and placed in Bouin’s solution. Measurements were 
then made of the number of antennal segments and head width. 
These data were used for determining the instars of live larvae 
through the fourth stage. 
Summarization of Data 
For each of the seven combinations the following two kinds of 
data were gathered: (1) mean number of days to the first appearance 
of an egg, 1st-, 2nd-, 3rd-, and 4th-stage larva, presoldier and 
soldier; and (2) mean caste composition of incipient colonies at 
selected intervals. Not all colonies were established nor observed on 
the same days. To make the various pairings comparable, dates of 
establishment and observation were converted to Julian dates. 
Observations were then grouped by the number of days past the 
data of pairing. Results of pairings replicated over 2 or 3 years were 
combined. To simplify data summarization, observations were 
grouped in 3-day intervals. Because of the different times of 
observation, not all of the colonies were included in each 3-day 
interval, giving rise to fluctuations in the number of colonies 
included in the summarized data. 
When both dealates had died, colonies were removed from the 
experiment. Some of the colonies remained viable for more than 2 
