APPENDIX II 
IX 
Appendix II: Chancroid Experiment Report Excerpt 
CHAHCSQXD SXPKRIU3HT 
Datei October 23, 1?4&# 
^ On Toady) October IS a tube of lyopldliaed Ducrsy bacillus dated 
9-20-48 MV. was opened, lcc of distilled water »u added end the naterial vie 
_ onto agar slants prepared^Sains the V!81L nedli* adjusted to /& 7 Jl to 
v.Au.->4/2 oo of defrltrineted fresh rabbits blood «u added Just prior to 
inoculation. The oatarial shoved £ood crcwth end on S atu r d a y . Ootober 23 at 
500 A.U. the cultures vera scraped off the slants into the ajpernatant blood, 
vidcli was transferred into a rial with sterile gla ss boadBjTthe- Material ua s 
; ihe natArlCTwas applism 
proxinately O.Olec 
first patimrt re coircil «e appl i ra- 
ti « last pa-dent received the aaterial 
at 705 A.H. » JhV Material van stub trad mid cultured following the last applies*- 
n mdthsea4 e ^fa vas fowl to la po sitive. patients J <r 
yUl agit ated to brvajt n> th e dlwjs of_bact?: 
i'froa a t2je?<rj33K syringe through a 25 
ftf — n +.q yj .1 ifM £iut nnin ft anh air 
tion of the natarial at 6*3o A.' 
tion anathe 
llntw— CiW< 
the negisrlito 
e f the fln as tta d a ii - 
■Sr 
f.*C3U 
used tos sufcjecte* 
and left era, outer aspects, and in the left s^pre scapular a 
was nade by a 20 .-auge hypoderaic i«edlb 
to draw blo->i. The right a_n aa-jub!it vu*> 
by ft^J^^fc-ieeirten and the abodider area., — — , . ■ - ■■ — _ 
soon os the s tr etch was aerfe ft 0,Glcc± of bV* Inoculum was placed oo the abraided 
area and i.jantly rubbed in by tae flat surface of triotl.ir nee £lft. The, patients 
On the right ana 
a a lA" scratch 
encrugh 
left 40^ 
not allowed to wash following the iaouulfctiJi^ sad -be x< anirt^wO’ no t put 
on until tha area had dried. At 1 how, 2 bo ... , and 4 hours after inoculation 
the prqpty l a x ia was applied as indicated •> tha aos’lcsLaet. , 
jTre&h lynado up just bef ore uac|LiU> X3Q3 cc of 
10 gg, of Draft "\ ^i )J ) gcs. of taapiiarsen we 
^applied u« ^iSv^d'bailT^r tas ''Jf nlTcd in icr 30 seoonda # 
using the aaterial that reaainoi on the glora w.ich was frequently dippod into 
the solution* ib* ztQA pro was applied jy lea# b ev i tes r wed h e ap. to the right 
the left am was treated by the standard U.5. A rev Pro-Eit^ I ten So, 
9110000, Lot So. V 45^ being rubbed in for 30 sc solids by the clcrcd hand of the 
physicians fog . v Vwfl a ^j sa d ew^bsr) . Bach patient received s i multa ne ous applica- 
Any j 
tics* of the Arwy prop^yluctie for the 
tine interval listed below. 
length of tiae and at- the 
600 
705 
700 
7«53 
3i53 
9t0& 
U«15 
11 137 
Inoculation started 
Inoculation coexisted 
Propiylaxis started for first group) So. 1 through 41# 
* findfthftri 
Prophylaxis started on second group - 2 hours after inoculation 
Prophylaxis oocpleted an second groip - Has. 42 through 83. J . 
Propiyisxia started on third group - 3 hours after inoculation. 
Propi^laxia finished - Kos. 55 through 133a 
Chancroid Experiment. Report. PCSBI HSPI Archives, CTLR-0000978. 
141 
