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Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators 
The research proved that raising the supply temperature, which lowers the relative humidity, not 
only reduces ammonia production but provides conditions closer to the thermoneutral zone for 
mice. Analyzed supply temperatures of about 22 °C (72 °F) were found to result in room 
temperatures between 24 and 26 °C (75 to 79 °F) with cages around 2 °C (4 °F) higher. While 
this is within the preferred temperature range for mice, it may prove too warm for scientists. 
