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Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators 
How To Use This Document 
The document is in two volumes. Volume I describes the project and its findings, appendix I in 
volume I presents relevant experimental data, such as experimental and calibration procedures 
and tabulated experimental data, and volume II presents summary data for more than 100 animal 
research facility configurations. 
Volume I is divided into eight major sections: 
1. Introduction 
5. Computational Fluid Dynamics Section 
2. Animal Room Variations 
6. Statistical Analysis 
3. Evaluation of Results and Recommendations 
7. Glossary of Relevant Terms 
4. Establishing and Verifying the CFD Methodology 
8. References 
Section I: Provides background information that will aid in understanding the project 
objectives and scope. This should be read by all. 
Section II: Outlines the computational fluid dynamics (CFD) base line model, including such 
details as the physical geometry chosen, details of cages, racks, and change stations. This section 
also includes a table summarizing the description of 100 animal research facilities considered in 
this project. It outlines the different means of presenting the vast data generated. It offers 
explanations of a qualitative approach to visualizing the flow (vector plots, fill plots) and of the 
quantitative analysis parameters such as mean temperatures, CO 2 and NH3 concentrations in the 
in the room and cage breathing zones. The results from the base line simulation are presented 
using these two approaches. 
Section III: Gives a graphical presentation of results for all 100 animal research facilities for 
mean room breathing zone temperature, mean cage temperature, mean room breathing zone 
relative humidity, mean cage relative humidity, room breathing zone CO 2 concentration, and 
mean cage CO 2 concentration. It also presents room breathing zone and mean cage NH3 
concentrations on a given day within the 10-day cycle (day 4). The section includes conclusions 
and recommendations for the following: supply type and exhaust position; air change rate; 
change station design and status; pressurization of room relative to corridor; orientation of cage 
racks and rack groupings; density of cages; side cracks of cages sealed instead of open; room 
width; and supply temperature. Finally, this section presents NH3 levels in the cages and 
breathing zone for all the 100 animal research facilities over the 10 days of the cycle. 
Section IV : Contains experimental data and validation of the computational fluid dynamics 
methodology used in this project. This section identifies and explains the experimental tests and 
procedures for the cage conditions, CO2, NH3, H2O, and heat generation for mice. In this section 
the validation of CFD vs. experimental work as well as diffuser validation against 
manufacturer’s data are presented. 
