Volume I - Section I - Introduction 
Page 1-17 
• Study III: 
Temperature: No significant temperature differences were observed when pine 
shavings were used instead of hardwood chips. 
Carbon dioxide: There were statistically significant (P < 0.05) elevations in CO 2 
levels in all caging systems when pine shavings were used. The molded polyester 
system averaged 1,157.8 ppm versus 967.9 ppm with hardwood chips. The 
Microisolator and Micro-Barrier systems averaged 3,485.9 ppm and 3,651.6 ppm with 
pine shavings versus 3,187.5 ppm and 3,616.1 ppm with hardwood chips. Trends 
among caging systems were similar to those observed when hardwood chips were 
used. 
Relative humidity: There were similar trends to studies I and 13 in RH and no 
significant differences were observed when shavings were used. 
Ammonia: There were similar trends to studies I and II and no significant differences 
in NH3 levels were observed when pine shavings were used. 
• Study IV: 
Temperature: No significant differences in temperature were detected when the CD-I 
stock was used instead of the DBA/2J strain. 
Carbon dioxide: There were statistically significant (P < 0.05) elevations in CO 2 
levels when the CD- 1 stock was used in the Microisolator and Micro-Barrier caging 
systems. The Microisolator system averaged 4351.8 ppm versus 3187.5 ppm with the 
DBA/2J strain. The Micro-Barrier system averaged 4376.8 ppm versus 3616.1 ppm 
with the DBA/2J strain. Trends among caging systems were similar to those noted 
when the DBA/2J strain was used. 
Relative humidity: There were significantly higher humidity levels (P < 0.05) within 
the Microisolator cage type with the CD-I stock (79.5 percent RH vs. 69.7 percent 
RH). Trends among caging systems were similar to those noted when the DBA/2J 
strain was used. 
Ammonia: Trends in ammonia levels among caging systems were similar to those 
noted when the DBA/2J strain was used. No significant differences were observed. 
• Study V: 
No significant differences were observed between polycarbonate systems having 
