Volume I - Section I - Introduction 
Page 1-27 
• Cage air velocities 
• Cage ventilation rate 
• Cage temperature rise 
The measurements were taken for: 
• Different cage orientations 
• With and without simulated mice 
• Different cage configurations and designs 
• L/min CO 2 injection rate using infrared analyzers 
• 100 mL/min CO 2 injection rate using infrared analyzers 
• 100 mL/min SF 6 injection rate using gas chromatography 
All combinations of approach velocity and cage configuration were simulated in several hundred 
CFD simulations with appropriate boundary conditions specified to ensure the CFD model 
agreed with the measured data. This work is documented in section 4. 
A further set of extremely careful experiments was undertaken with outbred female mice of 
HSD-ICR strain, five per cage on hardwood (Beta chip) shavings as bedding. Three indirect, 
convective calorimeters were used for this phase of project. Air temperature, velocity, and 
relative humidity were controlled in each calorimeter. The calorimeters were used to measure 
mice heat, and CO 2 and NH3 mass production for different cage humidity conditions. This gave 
constant CO 2 generation rates but showed that NH3 generation rose over the 10-day period of the 
experiments and depended on the relative humidity level. This meant the CFD data needed to be 
post-processed to reflect a room’s performance over a 10-day period. 
A third series of experiments were carried out to measure the following factors at several 
thousand points in an “empty” room using a sampling frequency at each point of 40 Hz.: 
• Average velocity 
• Minimum velocity 
• Maximum velocity 
• Turbulence intensity 
Further measurement of boundary conditions was carried out in an “occupied” room with racks, 
cages, and simulated animals. At each point the following were measured: 
• Mean air velocity 
• Median air velocity 
• Turbulence intensity 
• Air velocity fluctuation range 
