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Ventilation Design Handbook on Animal Research Facilities Using Static Microisolators 
High RH Level: 
CO 2 generation rate (g /hr/ lOOg BW) = 8.75e-l g/ hr/ lOOg BW 
H 2 O generation rate (g /hr/ lOOg BW) = 8.78e-l g / hr/ lOOg BW 
It can be noted that the generation rates of H 2 O and CO 2 do not change significantly with the RH 
level. We can therefore further reduce the generation rates to two numbers, which we can regard 
as constant irrespective of RH or day in the experiment. 
CO 2 generation rate (g /hr/ lOOg BW) = 9.05e-l g / hr/ lOOg BW 
H 2 O generation rate (g /hr/ lOOg BW) = 8.3 le-1 g/ hr/ lOOg BW 
Table 4.1.04 Tabular Variation of RH, NH 3 and NH 3 (max) with Day: Test 1, Lights Off 
Cage 
Group 
Day 
RH actual (percent) 
nh 3 
(g/hr/lOOg BW) 
NH3 (max) 
(g/hr/100g BW) 
1-4 
(Desired 
RH = 
30-35 
percent) 
1 
71.3 
4.69E-04 
5.87E-04 
2 
66.1 
5.44E-04 
7.32E-04 
3 
70.4 
8.30E-04 
9.96E-04 
4 
62.9 
1.26E-03 
1.52E-03 
5 
61.4 
0.00E+00 
2.07E-04 
6 
54.4 
1.34E-03 
1.38E-03 
7 
52.6 
1.96E-03 
2.22E-03 
8 
58.4 
1.86E-03 
2.06E-03 
9 
61.4 
4.31E-03 
4.55E-03 
10 
57.1 
5.62E-03 
6.28E-03 
5-8 
(Desired 
RH = 
75-80 
percent) 
1 
69.7 
3.88E-04 
4.76E-04 
2 
84.8 
5.23E-04 
6.49E-04 
3 
83.8 
1.46E-03 
1.91E-03 
4 
78.2 
3.73E-03 
4.11E-03 
5 
77.7 
4.06E-03 
4.68E-03 
6 
79.6 
4.27E-03 
4.48E-03 
7 
76.3 
5.22E-03 
5.39E-03 
8 
81.1 
7.30E-03 
7.65E-03 
9 
83.4 
6.60E-03 
6.70E-03 
10 
80.6 
5.02E-03 
5.22E-03 
9-12 
(Desired 
RH = 
30-35 
percent) 
1 
68.0 
5.67E-04 
6.38E-04 
2 
67.7 
5.24E-04 
7.59E-04 
3 
62.8 
6.99E-04 
8.39E-04 
4 
66.2 
1.23E-03 
1.44E-03 
5 
56.8 
1.29E-03 
1.42E-03 
6 
55.5 
1.59E-03 
1.65E-03 
7 
62.4 
2.59E-03 
2.69E-03 
8 
54.1 
2.04E-03 
2.24E-03 
9 
60.2 
4.71E-03 
4.78E-03 
10 
60.5 
3.43E-03 
4.01E-03 
