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Design Handbook on Animal Research Facilities Using Static Microisolators 
Calorimeter #1 
Time (min) 
Different wt d 
(g) 
pco 2 e 
(L/min) 
P 0 2 r (L/min) 
RQ* 
0 2 Recovery 
Ratio 
(percent) 
co 2 
Recovery 
Ratio 
(percent) 
10 
0.68 
0.0662 
0.0993 
0.74 
95.58 
105.37 
20 
0.66 
0.0642 
0.0963 
0.74 
98.47 
108.56 
30 
0.66 
0.0642 
0.0963 
0.70 
103.40 
108.56 
40 
0.67 
0.0652 
0.0978 
0.70 
101.85 
107.67 
50 
0.67 
0.0652 
0.0978 
0.71 
101.85 
108.40 
60 
0.67 
0.0652 
0.0978 
0.75 
97.00 
108.40 
70 
0.67 
0.0652 
0.0978 
0.71 
101.85 
108.40 
Calorimeter #2 
Time (min) 
Different wt d 
(g) 
pco 2 e 
(L/min) 
P 0 2 f (L/min) 
RQ* 
0 2 Recovery 
Ratio 
(percent) 
co 2 
Recovery 
Ratio 
(percent) 
0 
10 
0.69 
0.0671 
0.1007 
0.70 
104.26 
110.22 
20 
0.68 
0.0662 
0.0993 
0.70 
105.79 
111.84 
30 
0.69 
0.0671 
0.1007 
0.70 
109.22 
110.22 
40 
0.71 
0.0691 
0.1036 
0.67 
106.15 
107.84 
50 
0.67 
0.0652 
0.0978 
0.68 
112.48 
114.27 
60 
0.70 
0.0681 
0.1022 
0.68 
107.66 
109.38 
70 
0.69 
0.0671 
0.1007 
0.68 
109.22 
110.96 
Calorimeter #3 
Time (min) 
Different wt d 
(g) 
pco 2 e 
(L/min) 
P0 2 f (L/min) 
RQ* 
0 2 Recovery 
Ratio 
(percent) 
co 2 
Recovery 
Ratio 
(percent) 
0 
0.80 
10 
0.62 
0.0603 
0.0905 
0.77 
88.40 
101.44 
20 
0.59 
0.0574 
0.0861 
0.81 
88.25 
106.60 
30 
0.61 
0.0594 
0.0890 
0.73 
94.35 
103.11 
40 
0.59 
0.0574 
0.0861 
0.81 
88.25 
106.60 
50 
0.59 
0.0574 
0.0861 
0.70 
102.19 
107.99 
60 
0.60 
0.0584 
0.0876 
0.70 
100.49 
106.19 
70 
0.60 
0.0584 
0.0876 
0.70 
100.49 
106.19 
a. C0 2 generation rate based on C0 2 concentration and air exchange flow rate. 
b. 0 2 generation rate based on 0 2 concentration and air exchange flow rate. 
c. Weight of ethanol for every 10 minutes. 
d. Different weight of ethanol based on 10 minutes /production of C0 2 due to burning ethanol. 
e. C0 2 generation rate based on burning ethanol. 
f. 0 2 generation rate based on burning ethanol. 
g. Respiration quotient. 
