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Design Handbook on Animal Research Facilities Using Static Microisolators 
Date: OCT 21,1997 Day: 4 
Barometric pressure: 30. 1 8 (in) Desired temperature: 24+- 1 .5 °C 
Desired airflow: 5 (1/min) 
Cage: 1,2,3, 4 Calorimeter - 1 (desired RH 30-35 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.1 
0.58 
13 
41 
4.99 
75.74 
24.30 
53.50 
2 
20.0 
0.58 
17 
41 
4.99 
77.54 
25.30 
52.27 
3 
20.0 
0.59 
22 
41 
4.99 
77.18 
25.10 
56.60 
Average 
20.0 
0.58 
17 
41 
4.99 
76.82 
24.90 
54.12 
4 
19.9 
0.78 
15 
41 
4.99 
77.18 
25.10 
61.98 
5 
19.9 
0.81 
27 
41 
4.99 
75.92 
24.40 
62.71 
6 
20.0 
0.85 
28 
41 
4.99 
76.64 
24.80 
64.03 
Average 
20.0 
0.81 
23 
41 
4.99 
76.58 
24.77 
62.91 
Mice weight=420.3 (g) 
Cage: 5, 6,7 ,8 Calorimeter - 2 (desired RH 75-80 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.1 
0.55 
29 
39.5 
4.96 
73.73 
23.18 
74.05 
2 
20.0 
0.55 
40 
39.0 
4.89 
75.03 
23.91 
72.41 
3 
20.1 
0.55 
47 
39.0 
4.89 
74.97 
23.87 
74.70 
Average 
20.1 
0.55 
39 
39.2 
4.91 
74.58 
23.65 
73.72 
4 
20.1 
0.67. 
59 
39.0 
4.89 
75.47 
24.15 
79.25 
5 
20.1 
0.67 
75 
39.0 
4.89 
76.45 
24.69 
78.26 
6 
20.1 
0.66 
78 
39.5 
4.96 
77.56 
25.31 
77.23 
Average 
20.1 
0.67 
71 
39.2 
4.91 
76.49 
24.72 
78.25 
Mice weight=424.7 (g) 
Cage: 9,10,11,12 Calorimeter - 3 (desired RH 30 -35 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.1 
0.56 
8 
44 
4.95 
73.40 
23.00 
61.25 
2 
20.1 
0.58 
15 
44 
4.95 
74.93 
23.85 
61.65 
3 
20.1 
0.57 
21 
44 
4.95 
72.64 
22.58 
64.94 
Average 
20.1 
0.57 
15 
44 
4.95 
73.66 
23.14 
62.61 
4 
20.3 
0.63 
16 
44.0 
4.95 
73.74 
23.19 
64.76 
5 
20.1 
0.65 
28 
43.5 
4.86 
75.19 
23.99 
65.74 
6 
20.1 
0.73 
28 
44.0 
4.95 
76.70 
24.83 
68.04 
Average 
20.2 
0.67 
24 
44.0 
4.92 
75.21 
24.01 
66.18 
Mice weight=437.1 (g) 
