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Design Handbook on Animal Research Facilities Using Static Microisolators 
Date: DEC 16,1997 Day: 4 
Barometric pressure: 29.94 (in) Desired temperature: 24+-1.5 °C 
Desired airflow: 5 (1/min) 
Cage: D«3,4 Calorimeter - 1 (desired RH 30-35 percent) 
Concentration 
Air! 
low 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.1 
0.54 
2 
42.0 
4.89 
76.69 
24.83 
57.36 
2 
20.1 
0.55 
2 
42.5 
4.97 
76.83 
24.91 
56.05 
3 
20.1 
0.57 
2 
42.0 
4.89 
76.84 
24.91 
57.89 
Average 
20.1 
0.55 
2 
42.2 
4.92 
76.79 
24.88 
57.10 
4 
19.9 
0.81 
5 
42.0 
4.89 
77.57 
25.32 
62.12 
5 
19.9 
0.79 
6 
42.0 
4.89 
77.24 
25.13 
63.67 
6 
19.9 
0.79 
6 
42.0 
4.89 
76.95 
24.97 
65.31 
Average 
19.9 
0.80 
6 
42.0 
4.89 
77.25 
25.14 
63.70 
Mice weight=437.4 (g) 
Morning: Nt 
3 (tube)=l ppm Afternoon: NH 3 (tube)=lppm 
Cage: 5, 6, 7, 8 
Calorimeter - 2 (desired RH 75-80 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.0 
0.58 
2 
42.5 
5.04 
76.30 
24.61 
77.94 
2 
19.9 
0.60 
2 
42.0 
4.96 
76.19 
24.55 
78.99 
3 
20.0 
0.60 
2 
42.0 
4.96 
76.19 
24.55 
79.60 
Average 
20.0 
0.59 
2 
42.2 
4.98 
76.23 
24.57 
78.84 
4 
19.9 
0.72 
6 
42.0 
4.96 
76.38 
24.66 
83.70 
5 
19.9 
0.72 
7 
42.0 
4.96 
75.96 
24.42 
83.32 
6 
20.0 
0.74 
8 
42.0 
4.96 
75.89 
24.38 
84.44 
Average 
19.9 
0.73 
7 
42.0 
4.96 
76.08 
24.49 
83.82 
Mice weight=427.1 (g) Morning: Nf 
3 (tube) = 
.5 ppm A] 
temoon: N 
[H 3 (tube) = 8 ppm 
Cage: 9,10,11,12 Calorimeter - 3 (desired RH 75-80 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.0 
0.59 
2 
44.5 
5.27 
74.28 
23.49 
73.42 
2 
20.1 
0.59 
2 
43.0 
5.03 
74.45 
23.58 
73.37 
3 
19.9 
0.62 
3 
43.0 
5.03 
75.21 
24.01 
75.35 
Average 
20.0 
0.60 
2 
43.5 
5.11 
74.65 
23.69 
74.05 
4 
19.8 
0.82 
8 
43.0 
5.03 
74.50 
23.61 
78.59 
5 
19.7 
0.83 
10 
43.0 
5.03 
74.28 
23.49 
78.49 
6 
19.7 
0.85 
12 
43.0 
5.03 
75.29 
24.05 
81.24 
Average 
19.7 
0.83 
10 
43.0 
5.03 
74.69 
23.72 
79.44 
Mice weight=433 (g) Morning: NH 3 (tube )=8 ppm Afternoon: NH 3 (tube)=10 ppm 
