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Design Handbook on Animal Research Facilities Using Static Microisolators 
Date: DEC 19,1997 
Barometric pressure: 29.78 (in) 
Desired airflow: 6 (1/min) 
Day: 7 
Desired temperature: 24+-1.5°C 
Cage: 1 ,2,3*4 Calorimeter - 1 (desired RH 30-35 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.1 
0.50 
10 
48.0 
5.83 
75.27 
24.04 
49.90 
2 
20.1 
0.50 
49.0 
5.99 
75.29 
24.05 
56.10 
3 
20.1 
0.49 
49.0 
5.99 
75.32 
24.07 
57.26 
Average 
20.1 
0.50 
10 
48.7 
5.93 
75.29 
24.05 
54.42 
4 
20.1 
0.72 
8 
48.0 
5.83 
73.40 
23.00 
63.81 
5 
20.0 
0.73 
20 
48.0 
5.83 
74.76 
23.76 
63.47 
6 
20.0 
0.72 
24 
48.0 
5.83 
73.91 
23.28 
64.83 
Average 
20.0 
0.72 
17 
48.0 
5.83 
74.02 
23.35 
64.04 
Mice weig 
it=453 (g) Morning: NH 3 (tube)=2 ppm Afternoon: N 
Hh (tube )=2 ppm 
Cage: 5,6,73 
Calorimeter - 2 (desired RH 75-80 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.2 
0.45 
85 
51.0 
6.24 
76.56 
24.76 
73.80 
2 
20.1 
0.46 
89 
51.0 
6.24 
76.49 
24.72 
84.48 
3 
20.1 
0.46 
89 
50.0 
6.09 
76.61 
24.78 
87.43 
Average 
20.1 
0.46 
88 
50.7 
6.19 
76.55 
24.75 
81.90 
4 
19.9 
0.74 
122 
49.0 
5.94 
75.96 
24.42 
88.26 
5 
19.9 
0.75 
135 
49.0 
5.94 
76.38 
24.66 
87.53 
6 
19.9 
0.72 
147 
49.0 
5.94 
75.64 
24.24 
86.79 
Average 
19.9 
0.74 
135 
49.0 
5.94 
75.99 
24.44 
87.53 
Mice weig 
it=447.2 (g) Morning: NH3 (tube)=84 ppm Afternoon: NH3 (tube)=l 10 ppm 
Cage: 9,10,11*12 Calorimeter - 3 (desired RH 75-80 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
(1/min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.0 
0.46 
87 
48.0 
5.88 
74.97 
23.87 
72.07 
2 
20.0 
0.63 
89 
50.0 
6.18 
75.20 
24.00 
73.37 
3 
20.1 
0.52 
92 
49.0 
6.03 
75.02 
23.90 
72.31 
Average 
20.0 
0.54 
89 
49.0 
6.03 
75.06 
23.92 
72.58 
4 
19.9 
0.75 
139 
49.0 
6.03 
74.99 
23.88 
77.14 
5 
19.9 
0.73 
153 
49.0 
6.03 
74.95 
23.86 
77.23 
6 
19.9 
0.74 
149 
49.0 
6.03 
73.80 
23.22 
75.20 
Average 
19.9 
0.74 
14 
49.0 
6.03 
74.58 
23.66 
76.52 
Mice weight=449.3 (g) 
Morning: NH3 (tube )=86 ppm Afternoon: NH3 (tube)=105 ppm 
