Page A I - 76 
Design Handbook on Animal Research Facilities Using Static Microisolators 
Date: DEC 22,1997 Day: 10 
Barometric pressure: 29.73 (in) Desired temperature: 24+-1.5°C 
Desired airflow: 5 (1/min) 
Cage: 1,2,3, 4 
Calorimeter - 1 (desired RH 30-35 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
( 1 /min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.3 
0.23 
7 
69.0 
9.18 
72.66 
22.59 
50.33 
2 
20.3 
0.17 
25 
69.0 
9.18 
72.49 
22.49 
50.14 
3 
20.4 
0.38 
30 
69.0 
9.18 
72.50 
22.50 
44.51 
Average 
20.3 
0.26 
21 
69.0 
9.18 
72.55 
22.53 
48.33 
4 
20.3 
0.37 
15 
69.0 
9.18 
72.71 
22.62 
51.42 
5 
20.3 
0.37 
35 
69.0 
9.18 
72.66 
22.59 
52.42 
6 
20.3 
0.35 
43 
69.0 
9.18 
72.67 
22.59 
53.29 
Average 
20.3 
0.36 
31 
69.0 
9.18 
72.68 
22.60 
52.38 
Mice weig 
it=47 1.8 (g) Morning: NH 3 (tube)=l 1.5 ppm Afternoon: N 
(tube )=12 ppm 
Cage: 5,6,7$ Calorimeter - 2 (desired RH 75-80 percent) 
Concentration 
Airflow 
Actual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
( 1 /min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.3 
0.28 
117 
68.0 
8.80 
74.40 
23.56 
78.20 
2 
20.1 
0.27 
127 
68.0 
8.80 
74.98 
23.88 
81.17 
3 
20.1 
0.56 
145 
68.0 
8.80 
74.80 
23.78 
80.38 
Average 
20.2 
0.37 
130 
68.0 
8.80 
74.73 
23.74 
79.92 
4 
20.1 
0.36 
118 
68.0 
8.80 
74.64 
23.69 
73.70 
5 
20.1 
0.38 
139 
68.0 
8.80 
74.56 
23.64 
78.86 
6 
20.1 
0.41 
142 
69.0 
8.95 
74.63 
23.68 
80.61 
Average 
20.1 
0.38 
133 
68.3 
8.85 
74.61 
23.67 
77.72 
Mice weig 
it=463.7 (g) Morning: NH 3 (tube)=140ppm Afternoon: Nf 
3 (tube)=150 ppm 
Cage: 9,10,11,12 | Calorimeter - 3(desired RH 75-80 percent) 
Concentration 
Airflow 
Act ual temperature 
Time 
o 2 
(percent) 
co 2 
(percent) 
NH 3 (ppm) 
Scale 
Rate 
( 1 /min) 
Temp (°F) 
Temp(°C) 
Actual RH 
(percent) 
1 
20.1 
0.30 
85 
69.0 
9.09 
73.00 
22.78 
75.00 
2 
20.1 
0.43 
102 
69.0 
9.09 
74.23 
23.46 
75.59 
3 
20.1 
0.53 
98 
69.0 
9.09 
73.10 
22.83 
78.00 
Average 
20.1 
0.42 
95 
69.0 
9.09 
73.44 
23.02 
76.20 
4 
20.1 
0.51 
98 
69.0 
9.09 
72.83 
22.68 
80.27 
5 
20.0 
0.43 
121 
69.0 
9.09 
72.81 
22.67 
81.15 
6 
20.1 
0.40 
128 
69.0 
9.09 
72.85 
22.69 
81.68 
Average 
20.1 
0.45 
116 
69.0 
9.09 
72.83 
22.68 
81.03 
Mice weight=465.2 (g) 
Morning: NH 3 (tube )=86 ppm Afternoon: NH 3 (tube)=96 ppm 
