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Design Handbook on Animal Research Facilities Using Static Microisolators 
Light off Generation rate 
Day 
- ♦ 
O 2 (I/min) 
CO 2 (1/min) 
C0 2 
(g/hr/lOOg 
bw) 
nh 3 
(g/hr/lOOg) 
Max NH 3 
(g/hr/lOOg) 
Mice wt (g) 
1 
-3.62E-02 
3.62E-02 
1.06E+00 
4.69E-04 
5.87E-04 
401.6 
2 
-2.28E-02 
2.78E-02 
8.02E-01 
5.44E-04 
7.32E-04 
408.0 
3 
-2.51E-02 
2.88E-02 
8.20E-01 
8.30E-04 
9.96E-04 
413.9 
4 
-3.00E-02 
3.61E-02 
1.01E+00 
1.26E-03 
1.52E-03 
420.3 
5 
-8.15E-03 
3.1 IE-02 
8.54E-01 
0.00E+00 
2.07E-04 
429.5 
6 
-9.78E-03 
2.79E-02 
7.52E-01 
1.34E-03 
1.38E-03 
437.1 
7 
-3.83E-02 
3.75E-02 
1.00E+00 
1.96E-03 
2.22E-03 
441.1 
8 
-3.96E-02 
3.93E-02 
1.03E+00 
1.86E-03 
2.06E-03 
449.9 
9 
-2.55E-02 
2.31E-02 
6.06E-01 
4.31E-03 
4.55E-03 
449.7 
10 
-3.04E-02 
2.97E-02 
7.61E-01 
5.62E-03 
6.28E-03 
459.4 
For cages: 5, 6, 7, 8 
Desired temperature: 24+-1.5 °C Desired humidity: 75-80 percent 
Light on Generation rate 
Day 
O 2 (l/min) 
C0 2 (1/min) 
co 2 
(g/hr/lOOg 
bw) 
nh 3 
(g/hr/lOOg) 
Max NH 3 
(g/hr/lOOg) 
Mice wt (g) 
1 
-3.04E-02 
2.80E-02 
8.06E-01 
1 .78E-05 
1.07E-04 
409.8 
2 
-2.76E-02 
2.69E-02 
7.73E-01 
3.78E-04 
6.49E-04 
410.1 
3 
-2.96E-02 
2.33E-02 
6.59E-01 
4.95E-04 
6.28E-04 
417.5 
4 
-2.46E-02 
2.41E-02 
6.68E-01 
2.04E-03 
2.48E-03 
424.7 
5 
-1.83E-02 
2.66E-02 
7.34E-01 
2.34E-03 
2.55E-03 
427.8 
6 
-3.96E-02 
3.30E-02 
8.97E-01 
- 
- 
433.2 
7 
-3.26E-02 
2.25E-02 
5.99E-01 
3.49E-03 
3.58E-03 
442.8 
8 
-2.43E-02 
2.72E-02 
7.14E-01 
4.26E-03 
4.44E-03 
449.4 
9 
-3.46E-02 
3.08E-02 
7.99E-01 
3.61E-03 
3.67E-03 
454.7 
10 
-2.35E-02 
2.21E-02 
5.65E-01 
3.46E-03 
3.53E-03 
461.1 
Light off Generation rate 
Day 
0 2 (1/min) 
C0 2 (1/min) 
C0 2 
(g/hr/lOOg 
bw) 
nh 3 
(g/hr/lOOg) 
Max NH 3 
(g/hr/lOOg) 
Mice wt (g) 
1 
-3.01E-02 
2.95E-02 
8.48E-01 
3.88E-04 
4.76E-04 
409.8 
2 
-3.33E-02 
3.07E-02 
8.82E-01 
5.23E-04 
6.49E-04 
410.1 
3 
-2.74E-02 
3.18E-02 
8.98E-01 
1.46E-03 
1.91E-03 
417.5 
4 
-2.29E-02 
2.83E-02 
7.86E-01 
3.73E-03 
4.11E-03 
424.7 
5 
-2.16E-02 
3.78E-02 
1.04E+00 
4.06E-03 
4.68E-03 
427.8 
6 
-1.20E-02 
3.87E-02 
1.05E+00 
4.27E-03 
4.48E-03 
433.2 
7 
-3.10E-02 
2.95E-02 
7.85E-01 
5.22E-03 
5.39E-03 
442.8 
8 
-4.13E-02 
3.77E-02 
9.89E-01 
7.30E-03 
7.65E-03 
449.4 
9 
-3.13E-02 
3.41E-02 
8.85E-01 
6.60E-03 
6.70E-03 
454.7 
10 
-3.02E-02 
2.65E-02 
6.76E-01 
5.02E-03 
5.22E-03 
461.1 
