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[Vol. 89 
deposited in growing oocytes, they become opaque; and ripe 
eggs are white. 
d. the presence or absence of corpora lutea in the bases of the 
ovarioles. These yellowish residues of nutritional cells remain in 
the ovaries when eggs have been laid. 
e. the presence or absence of a full or empty spermatheca. Individ- 
uals with no spermatheca or an empty spermatheca are incap- 
able of laying fertilized eggs which develop into workers or 
queens. An empty spermatheca appears as a small, transparent 
bladder on the common oviduct. When full of sperm, the sperma- 
theca is white and superficially resembles a ripe egg in size and 
coloration. 
Results: 
Our dissections enabled us to distinguish several physiologically 
different kinds of queens. To simplify the presentation of data, we 
Table 3. Number of Queenright and Queenless Nests of L. ambiguus and 
L. longispinosus Collected during June, July, and August and the Composition of 
their Broods 
L. ambiguus 
Queenless nests 
Queenright nests 
Type of Brood 
Type of Brood 
9, 
9, S, 
$ only 9 and $ and $ 
$ only 9 and $ 
and S 
June 
0 
5 0 
0 28 
0 
July 
0 
11 17 
1 37 
71 
August 
2 
9 8 
2 32 
77 
L. longispinosus 
Queenless nests 
Queenright nests 
Type of Brood 
Type of Brood 
9, 
9, 
S only 9 and $ and S 
$ only 9 and $ 
and $ 
June 
0 
0 1 
0 2 
1 
July 
0 
0 4 
0 1 
12 
August 
0 
0 1 
0 0 
3 
