CHEMICAL AND MICROSCOPICAL INVESTIGATIONS. 
The Quinine was associated with some Cinchonidine. My previous experiments were less satisfactory, 
perhaps owing to the great abundance of green colouring-matter found in this bark ; and perhaps, also, as 
regards the specimens from Loja, owing to the very immature state in which they were gathered. 
Change of Place of Groivth as affecting ■ Successive Generations of C. officinalis. 
Any addition to the amount of our knowledge on the effects of change of climate on these plants 
must be valuable to the cultivator, and I have no doubt that those so engaged will read with interest the 
following examination of three generations of one species, the C. officinalis of Linnaeus, growing respec- 
tively in South America, England (under glass), and in India. 
The original bark of the first generation, from the mountains of TTritusinga, near Loja (Peru), was sent 
to me, with the flowering branches and ripened seeds, by Don T. Biofrio, and from these I raised plants 
in 1859. This was fine-looking, but very much weathered Crown Bark, with few adherent lichens, it 
gave me : — 
No. 1. 
First Generation. 
Oxalate of Quinine . . . . .1-87 
Cinchonidine ...... P20 
Cinchonine . . . . . ‘04 
Total . . 3-11 
No. 2. 
Second Generation raised from the above Seed. 
No. 2 a. No. 2 b. 
Grown in England. Grown partly in England , partly in India. 
Sulphate of Quinine 
. 
. 1-36 
Oxalate of Quinine . 
. 1-40 
Cinclionicine (with. 
merely a 
trace of 
Quinine uncrystallized 
. -17 = 1-57 
Cinchonine) 
• 
. -57 
Cinclionicine 
•79 
Total 
. 1-93 
Total . . 2-36 
No. 
3. 
Third Generation, descended from No. 2 b. 
Sulphate of Quinine 
. 1-75 
Sulphate of Cinchonidine 
. 1-50 
Cinchonine 
. -08 
Total . . 3* *33 
In the third generation* it is easy to remark a sort of atavism , the produce having returned almost 
exactly to the first; and having, in the Neilgherries, in India, rather surpassed the quantity of alkaloid 
yielded by the first generation grown on the mountains of IJritusinga, its native habitat. 
This is so far very satisfactory, as showing that at least there is no deterioration in this species through 
the so great change of its acclimatization ; and I am strongly of opinion that experience will manifest a 
still further decided improvement, especially consequent upon the effects of mossing the bark.f 
* The analysis of this No. 3 I owe to Mr. Broughton. 
f A specimen from Mr. Thwaites, apparently of this sort, grown in Ceylon, gave me : — 
Sulphate of Quinine . . . . .3*93 
Quinine uncrystallized ..... 2*41 
6-34 
*51 
•28 
Cinchonidine 
Cinchonine . 
Total 
7*13 
