17,1 Schobl: Dried and Pulverized Vaccine Virus 57 
ments showed good results during the past four months, the 
procedure seems to be of practical use. 
In order to make the use of this vaccine as simple as possible, 
we have suggested that it be put up in ordinary straight, one 
cubic centimeter, amber glass vials with rubber stoppers sealed 
with paraffine, another vial of the same type to contain the glyc- 
erine necessary to dissolve the powder immediately before use. 
The vial is opened by removing the rubber stopper. The glyc- 
erine is poured into the vial containing the powder. The 
rubber stopper is tightly replaced and the contents are shaken 
for several minutes until the powder has mixed with the liquid. 
This simple and convenient way of putting up the dried vaccine 
may not be the best as far as preservation of the dried vaccina 
is concerned; sufficient moisture may penetrate into the vial 
to render the vaccine virus inert in less time than four months. 
It was therefore suggested that, in case the above-mentioned 
method will not give satisfactory results, the powder be kept 
in hermetically sealed ampules, or be kept on hand in open 
bottles placed in a small desiccator containing a hygroscopic 
chemical. 
Besides the experiments already mentioned, we have arranged 
a field experiment by shipping dried vaccine virus to various 
places in the Archipelago and back, and then testing its activity 
on monkeys by vaccination. The places to which dried vaccine 
was shipped and tested when returned to Manila are : Currimao, 
Ilocos Norte ; Pandan, Ilocos Sur; San Antonio, Zambales; Ca- 
lapan,, Romblon, Pasacao, Culion, Surigao, and Butuan ; Cagayan 
and Iligan ; Oroquieta and Dapitan ; Zamboanga, Jolo, Cotabato, 
Quinimi, Gian, Davao, Agutay, and Cuyo. The length of time 
necessary for shipping and reshipping was twenty-five days. 
The animals vaccinated with these dried vaccines showed first- 
class “takes.” 
The process of drying the vaccine pulp seems to decrease 
its bacterial content. In the experiments above mentioned the 
bacterial content decreased three hundred sixty times during 
the process of desiccating. 
It is hoped that this preparation will help a good deal in 
overcoming some of the difficulties with which health officers 
meet in eradicating smallpox in the Philippines. 
