386 
The Philippine Journal of Science 
1920 
actively motile amoebae. On forcibly opening the adherent coils 
of the intestine, a large collection of creamy pus was found 
inside the cavity formed by the adherent coils of the intestine. 
The pus cavity was found completely surrounded by the coils; 
no opening joining the cavity with the lumen of the intestine 
could be discovered. Smears of the pus showed numerous ac- 
tively motile amoebae. On staining the smears the pus was 
found to be free from other microorganisms. Several stained 
preparations were made from this pus. Another peculiarity 
found in the case was the presence of dysenteric lesions in the 
small intestine extending about 3 inches above the ileocoecal 
valve. Three typical dysenteric ulcers were found in this area. 
Lastly, an elongated, elevated, inflammatory patch was found on 
the peritoneal surface of the small intestine opposite the ulcers — 
the appearance being similar to that found in the large intestine. 
Smears from this patch showed an abundance of living amoebse, 
while smears from the other portions of the peritoneal surface 
of the intestine showed no amoebse. A portion of the affected 
small intestine and large intestine were removed for sectioning. 
CHARACTERS OF THE LIVING AMOEBA 
In fresh preparations made with pus taken from the pus 
cavity, numerous actively motile amoebse were found. As it was 
not possible to stain preparations at the time that this exam- 
ination was made, it was not suspected at the time that the 
amoeba differed altogether from the classical type, and accord- 
ingly no special attempt was made to make out any distinctive 
characters of the living amoebse. All that I remember was that 
the amoebse showed marked size differences. Some of them were 
vacuolated. The ectoplasmic and endoplasmic differentiation 
was notably marked. 
STAINED PREPARATIONS 
Stained preparations were made by making films, fixing them 
while still wet in acetic acid-picric acid solution and then stain- 
ing them by a modification of Dobell’s iron-hsematein method. 
The slides were stained overnight and then differentiated by 
ferrous alum solution under microscopic control. On cursory 
examination of these slides under the oil-immersion objective 
I noticed that the nucleus of the amoeba was not of the karyoso- 
mic type. On carefully examining a large number of individuals 
in the several preparations I made, I found the amoebse showed 
the following characters: 
