19,1 Wells: Tikitiki Extract for Beriberi 71 
One cubic centimeter of this extract equals 15 grams of tiki- 
tiki. The strength of the recovered alcohol will be from 20 
to 80 per cent by volume. This is diluted with water to make 
20 to 30 per cent alcohol and is used repeatedly. 
DOSE 
Ordinary dose: Three (3) teaspoonfuls daily. 
Serious cases: Double dose or more, according to requirement. 
This method was in use in the Bureau of Science until Jan- 
uary 1, 1916. The results obtained from the use of this product 
were so favorable that in 1916 it was decided to revise the method 
and to increase the production. During 1916 there was obtained 
a maximum possible production with the equipment at hand. 
Prior to this time one small copper still was utilized for con- 
centration. At this time a much larger still, which had been 
used for essential oil work, was put in operation for the first 
distillation. By the use of these two stills the production was 
brought to 6,687 bottles during 1916. During 1917 and 1918 
certain revisions in the methods of filtration made it possible 
to increase the production to 8,188 bottles in 1918. In 1919 
one small Elyria glass-enameled still and a Sharpies laboratory 
centrifuge were installed. By the aid of these units larger 
quantities were worked up with a quicker and more effective 
separation of the inactive substance and a greater concentration 
for the final product. Year by year the demand for the product 
has increased until, at the present time, the Bureau of Science 
is unable to supply more than a small fraction of the actual 
requirements of the distributors. 
The finished product is placed in 50 cubic centimeter bottles, 
which are sealed, pasteurized, and labeled at the Bureau of 
Science prior to delivery to the various organizations for dis- 
tribution. Such organizations as the Public Welfare Board, La 
Liga Nacional para la Proteccion de la Primera Infancia, and 
the Philippine Health Service regulate the distribution, in order 
to insure that treatment shall be administered to patients by com- 
petent physicians. 
PRESENT METHOD OF MANUFACTURE 
Rice polishings for use in this process must be free from 
insects, clean, and finely ground. The polishings from, freshly 
milled rice of a new crop are preferable. The tikitiki (the 
rice polishings) is extracted for a period of forty-eight hours 
in a solution of alcohol of 25 per cent by weight (determined by 
use of the Abbe immersion refractometer) . The proportion of 
