140 The Philippine Journal of Science isis 
PHYTOPHTHORA PABERI MAUBL. : THE CAUSE OF BUD ROT 
ISOLATION 
On March 11, 1919, isolations were made from the typical 
case of bud rot sent to the writer by the Bureau of Agriculture. 
The tree was about 4 years old and was approximately 3 meters 
tall. Infection had apparently started from the tip of the young 
unfolded leaves, and had advanced downward into the young 
tender tissue of the growing point and into the woody parts 
below. The bud rot was a typical case, such as has been de- 
scribed in the section on general diagnosis. The tree was split 
lengthwise. The diseased portion was 2 meters in length 
extending from the tip of the young leaves to 20 centimeters 
below the growing point. Microscopic examination of the in- 
fected parts of the leaves, 60 centimeters above the growing 
point, showed the presence of mycelia and chlamydospores. 
Portions were cut from the affected parts with a sterile scalpel 
and placed on prepared corn meal plates. A Phytophthora was 
isolated from the woody tissue below the growing point, from 
the parts adjacent to the growing point, and from the leaf 
sheath 90 centimeters above. A microscopic examination of 
the fungus indicated that it was apparently the same as Phy- 
tophthora faberi Maubl. from cacao. 
INFECTION STUDIES 
With coconuts. — Infection studies were immediately carried 
out with this Phytophthora. For the first experiments seedling 
coconuts, from 75 centimeters to 2.5 meters in height, were used. 
The stab method of inoculation, described under bacterial work, 
was employed. Because of the extremely dry weather, all in- 
oculated seedlings were placed in a damp chamber. Table III 
gives the results of the inoculations. 
