210 
The Philippine Journal of Science 
1919 
The other two corpuscles that were taken in by this individual 
were ingested in substantially the same manner, but the parasite 
was lost from the field in the sudden shifting of fiuid due to 
evaporation and could not again be found. In no instance did 
we observe the expulsion of a corpuscle that had been ingested. 
The presence of the projecting axostyle and the marginal un- 
dulating membrane placed the parasite in the trichomonad group, 
but it was not until specimens were stained that the five anterior 
flagella were discovered that enabled us to place the organism 
definitely in the genus Pentatrichomonas. Unfortunately, only 
two slides were prepared from this stool for staining, for it was 
assumed that the case would remain in the hospital and that an 
abundance of material could be procured for study. It was felt 
that the case was one of unusual interest, and efforts would have 
been made to secure an autopsy had the patient died in the 
hospital. 
At the time we made this observation, we believed that the 
ingestion of erythrocsrtes by an intestinal flagellate had not been 
reported previously; but subsequently we were able to obtain 
Chatterjee’s original paper on Pentatrichomonas {^) in which he 
says : “In several a full sized red corpuscle was found inside the 
body; no vacuole is seen.” We have been unable to find any 
development of this observation in the literature accessible to us. 
STUDY OF THE STAINED PREPARATIONS 
The films were fixed in Bouin’s picro-aceto-formol solution and 
stained by the alcoholic iron-hsematein method of Dobell. The 
slides were carefully searched with the aid of the mechanical 
stage, and we failed to find Entamoeba histolytica or any other 
protozoon in either the trophozoite or encysted stage. So far as 
concerns the parasite in question the results were rather dis- 
appointing, as is apt to be the case with preparations of the in- 
testinal flagellates. These parasites show a tendency to conceal 
themselves in the thicker parts of the film, and they only swim 
out into the open after the lapse of time. Relatively few were 
found. Fig. 1 shows an individual, found near the margiif of 
one film, that had unfortunately undergone some distortion 
through almost complete drying before the preparation was fixed. 
It was drawn because it contained a deeply stained disklike body 
that was, in all probability, an erythrocyte. Fig. 2 shows a 
fairly perfect individual, in which most of the parts are demon- 
strated. In no case did we succeed in demonstrating the mouth 
parts. Special methods such as are used in the demonstration 
of the neuromotor apparatus of ciliates and flagellates, such as 
