i6 
Colorado Experiment Station 
TABLE 17.— B. GOBI COMMUNIS 
Per 
cent. 
Honey 
Long-evity of 
Culture 
0 
5 10 
hrs. hrs. 
Id. 
2d. 
3d. 
4d. 
5d. 
6d. 
00 
9d. 
lOd. 
0.0 
+ 
4- 
4- 
4- 
4- 
4- 
4- 
4- 
4- 
4- 4- 
+ 
4- 
10.0 
+ 
4- 
4- 
4- 
4- 
± 
± - 
20.0 
+ 
4- 
4- 
4- 
4- 
+ 
— 
30.0 
+ 
4- 
4- 
4- 
4- 
— 
40.0 
+ 
4- 
+ 
4- 
4- 
— 
50.0 
+ 
4- 
4- 
4- 
± 
— • 
60.0 
+ 
4- 
4- 
4- 
— 
70.0 
-f 
4- 
4- 
4- 
± 
— 
80.0 
4- 
4- 
4- 
4- 
— 
90.0 
4- 
4- 
+ 
4- 
+ 
— 
100.0 
+ 
+ 
4- 
4- 
4- 
± 
± 
— 
As in the preceding series, B. coli communis was the most 
resistant organism studied so far as the higher concentrations 
were concerned. It did not die out in the pure honey until the 
fifth day, altho there was a reduction in numbers on the third day. 
On the second day there was a reduction in numbers in all con- 
centrations above 40 per cent., while by the third, all were dead 
above 20 per cent. The 20 per cent, died out by the fourth day, 
and the 10 per cent, was sterile on the sixth. The control was 
alive after 10 days. 
TABLE 18. — B. DYSENTERIAE 
Per 
Longevity of Culture 
cent. 
5 10 
Honey 
0 hrs. hrs. Id. 2d. 3d. 4d. 5d. 6d. 7d. 8d. 9d. lOd. 
0.0 
4-4-4-4-4-4-4-4-4"4-4-4-4- 
10.0 
4- ± — 
20.0 
4- ± — 
30.0 
4- — 
40.0 
4- — 
50.0 
4- — 
60.0 
4- — 
70.0 
4- — 
80.0 
+ — 
90.0 
4- — 
100.0 1 
4- 4- — 
B. dysenteriac appeared to be almost as sensitive as B. fecalis 
alkdi^cncs. It was present in the pure honey after 5 hours, but 
absent after 10. In 5 hours the organisms had disappeared from 
all dilutions above the 20 per cent, and the numbers were much 
reduced in the 10 and 20 per cent. ; after 10 hours, these, too, were 
sterile. The control gave growth after 10 days. 
