14 Colorado Experiment Station 
TABLE 13.— B. PROTEUS VULGARIS 
Per 
cent. 
Honey 
Longevity 
of 
Culture 
0 
5 
Ors. 
10 
hrs. 
Id. 2d. 
3d. 4d. 5d. 
6d. 7d. 8d. 9d. lOd. 
0.0 
+ 
+ 
+ 
+ + 
+ 
+ 
+ 
+ + + + + 
10.0 
+ 
+ 
+ 
+ + 
+ 
+ 
+ 
+ + + + + 
20.0 
+ 
+ 
+ 
+ + 
+ 
+ 
+ 
+ + + + + 
30.0 
+ 
+ 
+ 
+ — 
40.0 
+ 
+ 
+ 
— 
'0.0 
+ 
+ 
+ 
— 
60.0 
+ 
+ 
+ 
— 
70.0 
+ 
+ 
-f- 
— 
80.0 
+ 
+ 
+ 
— 
90.0 
+ 
+ 
+ 
— 
100.0 
+ 
+ 
+ 
+ + 
— 
B. pro tens vulgaris 
was 
the most resistant of all the organisms 
in the lowest concentrations, being present in apparently undimin- 
ished numbers in the control, 10, and 20 per cent, after 10 days. 
It was dead in the pure honey on the fourth day; after 24 hours 
it had disappeared from all dilutions above the 30 per cent., which 
was sterile on the second day. 
TABLE 14. — B. SUIPESTIFER 
Per 
cent. 
Honey 
Longevity of 
Culture 
5 10 
0 hrs. hrs. 
Id. 2d. : 
3d. 
4d. 5d. 
6d. 
7d. 8d. 
9d. 
lOd. 
0.0 
+ 
+ 
+ 
+ 
+ 
+ 
+ + 
+ 
+ + 
+ 
+ 
10.0 
+ 
+ 
+ 
+ 
+ 
± 
— 
20.0 
+ 
+ 
+ 
+ 
— 
30.0 
+ 
+ 
-h 
+ 
— 
40.0 
+ 
+ 
+ 
— 
50.0 
+ 
+ 
+ 
— 
60.0 
+ 
+ 
— 
70.0 
+ 
+ 
— 
80.0 
+ 
+ 
— 
90.0 
+ 
+ 
— 
100.0 
+ 
+ 
+ 
+ 
— 
In the ])ure honey, B. suipestifer was still alive on the second 
day, altho decreased in numbers, but by the third it was dead. In 
dilutions above 50 per cent, it was dead after 10 hours ; it was 
absent in 24 hours from the 40 and 50 per cent., and in 48 hours 
it had disappeared from the 20 and 30 per cent. On the third day 
the numbers were considerably reduced in the 10 per cent., and 
on the fourth day the solution was sterile. The control was alive 
after 10 days. 
