10 
Colorado Experimlnt Station 
fourth day. On the third day it disappeared from the 60, 70, 80, 
and 90 per cent., and on the fourth, fifth, and eleventh days from 
the 50 per cent., 40 per cent., and 30 per cent., respectively. Abund- 
ant growth was obtained from the control, 10 per cent., and 20 per 
cent, after 25 days, but in 40 days all were dead but the control. 
TABLE 6.— BACILLUS PARATYPHOSUS “B” 
Per 
cent. 
Honey 
Longevity of Culture 
0 
5 10 
hrs. hrs. 
Id. 
2d. 
3d. 4d. 5d. 40d. 
0.0 
+ 
+ 
+ 
+ 
+ 
+ + + + 
10.0 
+ 
+ 
+ 
+ 
± — 
20.0 
+ 
+ 
+ 
+ 
+ 
— 
30.0 
+ 
+ 
+ 
+ 
— 
40.0 
+ 
+ 
+ 
— - 
50.0 
+ 
+ 
+ 
— 
60.0 
+ 
+ 
± 
— 
70.0 
+ 
+ 
— 
80.0 
+ 
+ 
± 
— 
90.0 
+ 
+ 
— 
100.0 
+ 
+ 
+ 
+ 
Bacillus paratyphosus ''B” was among the most sensitive 
orgDiiisms studied. After 10 hours its numbers were very much 
r-rured in the 60, 70, 80, and 90 per cent. In 24 hours it had dis- 
appeared altogether from dilutions above 30 per cent.; no growth 
was obtained from the 30 per cent, on the second day, and on the 
third the organisms had died out in the 20 per cent, and were 
greatly diminished in the 10 per cent., from which it was absent 
nn the fourth day. The control in salt solution was alive on the 
fortieth day. It disappeared from the pure honey in 48 hours. 
TABLE 7.— BACILLUS COLI COMMUNIS 
Per 
Longevity of 
Culture 
cent. 
5 
10 
Honey 
0 
hrs. 
hrs. 
Id. 
2d. 
3d. 
4d. 
5d. 
6d. 
7d. 
8d. 
9d. 
lOd. lid. 
12d. 40d. 
‘ATo 
+ 
+ 
+ 
+ 
+ 
+ 
4 
4 
4 
4 
~4 
'4 
4 
4 
4 4 
10.0 
+ 
+ 
+ 
+ 
+ 
4- 
4 
4 
4 
4 
4 
4 
± 
± 
— 
20.0 
+ 
+ 
+ 
+ 
+ 
+ 
4 
± 
+ 
30.0 
+ 
+ 
+ 
-f 
+ 
+ 
4 
— 
40.0 
+ 
+ 
+ 
+ 
+ 
+ 
± 
50.0 
+ 
+ 
+ 
+ 
+ 
+ 
— 
60.0 
+ 
+ 
+ 
+ 
+ 
-1- 
70.0 
+ 
+ 
+ 
+ 
+ 
■f 
— 
80.0 
+ 
+ 
+ 
+ 
+ 
+ 
— 
90.0 
+ 
+ 
+ 
+ 
+ 
4 
— 
100.0 
+ 
+ 
+ 
+ 
4 
± 
4; 
Bacillus coli communis was the most resistant form met with. 
No harmful action couhl be noted in any concentration until the 
fourth day, when the culture was dead in the 50, 60, 70, 80, and 
