8 
Colorado Experiment Station 
In the pure honey, B. typhosus remained alive for 48 hours. 
After 24 hours it was dead in dilutions above 50 per cent.; after 
48 hours it had disappeared from the 50 per cent.; after 3 days it 
was alive only in the 10 per cent., and in the salt solution control, 
and after 4 days it was present only in the control, where it was 
still alive after 40 days. 
TABLE 2.— BACILLUS FECALIS ALKALIGENES 
Per 
Longevity of Culture 
cent. 
5 
10 
Honey 
0 
hrs 
. hrs. Id 
2d. 40d. 
0.0 
+ 
-F 
+ + 
+ + 
10.0 
-F 
+ 
+ — 
20.0 
+ 
+ 
— 
30.0 
+ 
— 
40.0 
+ 
+ 
— 
50.0 
+ 
— 
60.0 
+ 
— 
70.0 
+ 
— 
80.0 
— 
90.0 
+ 
— 
100.0 
+ 
— 
B. 
fecalis alkaligenes appears to be the most sensitive of all 
the organisms studied. It was 
killed by all concentrations above 
50 per cent., i 
ncluding pure honey, inside of 5 hours and after 10 
hours remained alive only in the 10 per cent, and control ; at the 
end of 24 hours it was present only in the control, where it was 
still al 
ive 
after 40 days. 
TABLE 
3.— BACILLUS PROTEUS VULGARIS 
Per 
Longevity of Culture 
cent. 
5 
10 
Honey 
0 
hrs. hrs. Id. 
2d. 3d. 4d. 
5d. 6d. 7d. 8d. 9d. lOd. 15d. 20d. 25d. 40d. 
O.o 
+ 
+ + 
+ + + 
+ + + -1- + + + +4- + 
10.0 
+ 
+ 
+ + 
+ -f- + 
+ + + + + + + + + * 
20.0 
+ 
+ 
+ + 
+ + + 
+ + -h-t- + + + + -H * 
30.0 
+ 
+ 
+ + 
+ + + 
+ — 
40.0 
+ 
+ 
+ + 
+ — 
50.0 
+ 
-h 
+ + 
— 
60.0 
+ 
+ 
+ + 
— 
70.0 
+ 
+ 
+ + 
— 
80.0 
+ 
-h 
+ + 
— 
90.0 
+ 
+ + 
— 
100.0 
+ 
+ 
+ + 
-f + ± 
— 
Bacillus proteus vulgaris was present in all concentrations dur- 
ing the first 24 hours; it died out in the pure honey after the 
fourth day. On the second day it had disappeared from all dilu- 
tions above 40 per cent., and on the third day it was absent from 
* Discontinued. 
