4 
Colorado Experiment Station 
From Combs 
White" has isolated Bacillus A {Bacillus mesentericus f), Bad. 
acidiformans, and Saccharomyces roseus from combs. 
From Pollen and Intestine oe Healthy Honey Bees 
From the pollen and intestine of the bee, White" has separated 
a motile rod, designated Bacillus B described briefly as follows : 
Motile, Gmm negative rod which liquefies gelatin slowly; egg yellow 
pigment; alkaline reaction in litmus milk; acid but no gas from dextrose; 
slight acid production from maltose and mannite ; alkaline reaction with 
lactose, saccharose and levulose. 
From Comb Honey and Healthy Larvae 
The same author states that he has examined comb honey 
from a large number of sources and has found it to be quite 
uniformly sterile. Healthy larvae are likewise sterile. The writer 
has made similar examinations of comb honey from the local 
market and his findings confirm those of White. 
Upon Adult Bees 
White has noted Bacillus A {B. mesentericusf) , Bact. cyaueus 
{Micrococcus cyaneus) and Micrococcus C as occurring upon adult 
bees. 
Micrococcus G . — Non motile. Gram positive coccus; liquefies gelatin; 
produces acid from dextrose, lactose, saccharose, levulose, maltose, and man- 
nite, but no gas ; milk is coagulated and the casein is liquefied. 
In Intestine oe Healthy Honey Bee 
According to White, the temperature of the healthy honey 
bee approximates that of warm blooded animals, and many of the 
same bacteria that occur in the bee’s intestine are also found in 
the intestine of man and other animals. The number of species 
in any individual is comparatively small, altho the number of 
organisms may be large. 
The list includes : 
Bacillus cloacae 
Bacillus coli communis 
. Bact. mycoides 
Bacterium D. 
Saccharomyces F. 
Saccharomyces roseus 
Bacterium /).— Non-niotile rod, non-spore-forming anaerobe; does not 
liquefy galatin ; produces neither acid nor gas from dextrose. 
Bacillus E.— Motile rod; does not produce spores. Gram positive; liquefies 
gelatin slowly ; lemon yellow in color ; produces acid from dextrose, saccharose, 
mannite; reaction alkaline with lactose, levulose. and maltose. Milk coagu- 
lated and casein slightly digested. 
Bacillus cholerae suis 
Bacillus sugastricus 
Ps. fluorescens liquefaciens 
Bacillus B. 
Sacch' romyces G. 
