222 
Philippine Journal of Science 
1919 
our analytical work on the different brands of tikitiki extract as 
prepared in local drug stores and private laboratories. However, 
inspection of the results shows that extracts 1, 2, and 3 are of 
approximately equal concentration, while extracts 4 and 5 occur 
as much lower concentrations, this being shown by their specific 
gravity and the proportions of total solids they contain. The 
results of our analyses are summarized in Table I. 
Table I . — Results of analyses of samples of tikitiki extract. 
Bureau of 
Science, 
extract 1. 
Extract 2. 
Extract 3. 
Extract 4. 
Extract 6. 
Temperature °C-- 
28 to 26 
26 to 25 
30. 3 to 30. 1 
28. 5 to 30. 1 
29. 3 to 29. 6 
Specific gravity 
1.2800 
1. 2960 
1.2878 
1. 2264 
0. 9572 
Total solids per cent. 
53.23 
66. 20 
60.33 
25.63 
6. 40 
Reducing sugar (by direct 
reduction) per cent.. 
23. 70 
20.80 
24.96 
15.27 
1.39 
Disaccharides (saccharose 
by inversion).. per cent.. 
2. 56 
0. 30 
0. 65 
0.57 
0.17 
Mineral residue do 
4. 40 
3.32 
4.87 
0.76 
0. 19 
Total nitrogen by Kjel- 
dahl . percent.. 
2.008 
2.300 
1.640 
0.856 
0. 140 
Phosphorus as P206.do 
1. 70 
0.375 
2. 14 
0. 24 
0. 165 
Sulphur (total) do 
0.26 
0. 109 
1.11 
0.059 
0.001 
Amylolytic enzyme 
negative 
negative 
negative 
negative 
negative 
A striking fact in this table is that extract 3, which has a 
higher specific gravity than extract 1, has however a lower per- 
centage of total solids than that extract. This fact might be 
accounted for by the different methods used in removing the 
alcohol from the extract and by the extent of its dilution with 
water so as to obtain it at the desired concentration. Further- 
more, we have the anomalous condition shown in extract 5, in 
which a sample with 5.4 per cent of total solids shows, at 29.3° 
to 29.6° C., a specific gravity of 0.9572. This condition might 
be explained by the presence of an unremoved residuum of the 
alcohol that was used as a solvent. 
The determination of total solids in vacuum is undoubtedly 
the most accurate method for collecting such data; but, regret- 
tably, our electric pump was not in working order at the time 
we undertook this investigation and we were forced, therefore, 
to employ other methods to obtain our results. 
The amounts of reducing sugar and disaccharides are practi- 
cally proportional to the total solids. The proportion between 
them, however, is variable and depends upon the degree of their 
hydrolyzation. In extract 2, which was purposely hydrolyzed, 
the proportion of disaccharides is very small. 
