XV, 6 
Shaw : Campbellosphaera 
497 
Close to the posterior pole of the specimen are two half-grown 
specimens of an endophytic alga, of which one shows plainly 
in the photograph (Plate I, fig. 1). More mature specimens 
of Campbellosphaera on the same slide bear an abundance of 
these endophytes in many stages of development. 
OBSERVATIONS ON LIVING SPECIMENS 
The earliest record in my notes pertaining to this genus 
relate to observations on living material collected at Pasig on 
July 30, 1914. Being mixed with larger species it was desig- 
nated in the notes as “the smaller, spheroidal species of Volvox.” 
The notes under the above date are as follow: 
Collections of this morning show the smaller species with asexual re- 
production. Very large gonidia radially vacuolate. Nearly closed daughter 
spheres with four and eight large cells, probably gonidia, which appear 
to be [derived from] undivided marginal cells of the saucer or cup stage. 
[These] migrated inward and became distributed in the * * * sphere. 
No protoplasmic connections in mother sphere. Cells of daughter very 
elongate and compactly arranged. External wall of cells of mother sphere 
very strongly convex on the outer side. Cells of the anterior pole larger 
and farther apart and with much larger stigmata. 
Measurements of three living specimens were recorded under 
the same date: 
Specimen 1. — Coenobium, 430 ^ wide by 480 m long. Somatic cells, sphe- 
roidal, 7 a* in anterior, middle, and posterior parts of coenobium. Inter- 
cellular distances, 8 to 12 as mostly about 10 a*. Cells in an area 180 
A* square, 180. Estimated number of ceils in coenobium, 3,470. Gonidia 
spheroidal; 82 by 85 as 80 by 81 as 86 by 88 as 88 by 90 as 89 by 94 a*. 
Daughters, 2; (1) 77 by 100 as with four gonidia about 25 as forming a 
close group in one end; space about the daughter 90 by 106 (2) 77 by 
100 as with four gonidia about 25 a* each also forming a close group in 
the blunt end; space about the daughter, 115 by 130 Daughters rotating 
on polar axis. 
Specimen 2 . — 410 by 460 a*. Cells, about 7 a<- (in equatorial region 
somewhat less). Intercellular distances, about 8 to 10 a*. Counted twenty- 
six cells in an area 75 a* square. Estimate of number of somatic cells, 
2,635. Gonidia measured 42 as 52 as 64 as 64 as 64 as 72 a*, 57 by 74 n 
(about to divide). Daughter, 80 p- collapsed, with eight gonidia about 18 
M- in diameter. 
Specimen S . — 410 by 480 n. Cells, about 7 Intercellular space, 8 
to 10 a*. Cells counted in 75 by 75 p space, 24. Estimate of somatic cells, 
2,520. Gonidia; anterior circle, 50 by 60 as 54 by 60 as 46 by 48 as 54 
by 56 m; posterior circle, 72 as 72 a*, 62 by 70 as 58 by 66 a*. 
A record of the movement of the gonidia from the close cluster 
in the back of the newly closed embryo to a more scattered dis- 
tribution in the daughter coenobium was made on the same day. 
In two embryos, at about 11.30 in the morning, the gonidia were 
