514 
Philippine Journal of Science 
1919 
The earliest described and well-established European species 
of Volvox, V. globator Ehrenberg (’38) and V. aureus Ehrenberg 
(’38), were described at length by Cohn (’75), whose colored 
plate of V. globator has long been a classic for textbook and 
handbook illustration, and by Klein (’89 A and ’90), who made 
the largest contribution to the stock of pictures of V. aureus in 
various stages and phases. His figures include at least one, 
(’90) Plate 2, fig. 4, which is decidedly not of V. aureus, but 
of one of the megalogonidiate species. At about the same time 
Overton (’89) made a contribution to the knowledge of the life 
history of the then known European species of Volvox. More 
exact knowledge of the cell membranes of the somatic cells of the 
two European species was the result of the work of Meyer (’95 
and ’96) who incidentally gave us what information we have 
on his new species, V. tertius. His diagrammatic drawings of 
the cell membranes of V. globator and V. aureus are becoming 
classic by reproduction in handbooks. I never look at these 
«r 
drawings without feeling that it is improper to retain these 
two species in the same genus. 
A species with a close affinity to V. globator was described 
by Powers (’08) from Nebraska under the name V. perglobator. 
The somatic protoplasts of this species are highly stellate and 
connected with their neighbors. The same is true of another 
species described by West (’10) from Rhodesia under the name 
V. rousseleti. In this one the cells are smaller and more 
numerous. 
My own Philippine material 6 contains at least two species, 
both labeled with new names in my note books and in the al- 
bums containing their photomicrographs, which are more or less 
closely similar to V. globator, V. perglobator, and V. rousseleti. 
In 1914 my collection of slides contained two excellent gly- 
cerin mounts of Volvox aureus (labeled V. minor) marked 
“4-16-96.” The date indicates that the specimens were collected 
at Stanford University, California. They were under cover 
glasses sealed to the slides with Brunswick black which had 
cracked and become loosened. They had been fixed and stained 
with picro-nigrosin and the glycerin was slightly tinged with 
the picric acid. The staining had been very light. The speci- 
mens were mostly in beautiful condition after eighteen years 
under the covers, but the glycerin had partly escaped and was 
6 Some material collected in Borneo by Mary Strong Clemens contains 
a similar species. 
