CONTENTS xi 
Methods for the Microscopic Study of Bacteria^ 
IV. Methods and Media for Cultivation of Bacteria — 
Egg Media 232 
Milk and Litmus Milk 232 
Potato 232 
Hiss' Senii-soHd Medium 233 
Russell Glucose-lactose Medium 233 
kSaccharose-mannitol Medium 233 
Media for the Cultivation of Aciduric Bacteria 233 
Oleate Agar for the Influenza Bacillus 233 
Media for the Cultivation of Anaerobic Bacteria 234 
V. The Cultivation of Bacteria 235 
1 . Inoculation of Culture Media 235 
2. The Isolation of Pure Cultures of Bacteria 236 
3. The Incubation of Bacterial Cultures 245 
VI. The Study of Bacterial Cultures 246 
1. Growth in Solid Media 246 
2. Growth of Bacteria in Fluid Media 249 
The Voges-Proskauer Reaction 252 
CHAPTER X. 
Bacteriological Examination of Material From the Patient and the 
Cadaver. 
Material from the Living Subject 257 
Blood Cultures ' 257 
Bacteriological Examination of Cerebrospinal Fluid 258 
The Examination of Peritoneal, Pleural and Pericardial Fluids 259 
Pus 260 
Examination of Urine 260 
Examination of Feces 261 
Examination of Sputum, of Buccal and Pharyngeal Material 263 
Bacteriological Examination of the Eye 265 
Subacute Conjimctivitis 266 
Bacteriological Examination of the Ear and Nose 266 
The UtiUzation of Animals for Bacterial Diagnosis and Experimentation 268 
The Inoculation of Anunals 269 
Cutaneous Inoculation 270 
CHAPTER XI. 
Practical Sterilization, Antisepsis and Disinfection. 
Laboratory Sterilization 272 
Physical Agents 272 
1. Heat 272 
2. Live Steam 274 
3. Fractional Sterilization 274 
4. Boiling Water • 274 
Chemical Solutions 275 
Salts of Heavy Metals 275 
Oxidizing Solutions 276 
Phenols, Cresols 276 
Tincture of Iodine 277 
Boric Acid 277 
Immune Sera as Disinfectants 278 
Formaldehyde 278 
Essential Oils 278 
Soaps ' 278 
Testing and Standardizing Liquid Disinfectants 279 
Gaseous Disinfectants 279 
1. Formaldehyde 279 
2. Paraform 280 
3. Sulphur 281 
4. Chlorine Gas 281 
5. Ozone 282 
