X CONTENTS 
Lysins — 
The Kahn Test in the Diagnosis of Syphihs — 
Shaking and Addition of Sahne 179 
Reading Results 179 
Interpretation of Results 179 
Recording Results 180 
Procedure With Less Than Three Tubes 180 
Quantitative Procedure With Scrum 181 
Qualitative Spinal Fhiid Procedure 182 
Quantitative Spinal Fluid Procedure 1 83 
Relationship Between Kahn and Wassermann Test 184 
Aggressins 185 
Opsonins. Tropins. Bacterial Vaccines 186 
Procedure 186 
1. Leukocyte Suspension 186 
2. Suspension of Bacteria 187 
3. Serum 187 
The Test 187 
The Nature of Opsonins 188 
Vaccine Therapy 189 
Prophylactic Vaccination 189 
Preparation of Vaccines 189 
The Injection 191 
The Dosage and Frequency of Injection 191 
Indications for the Use of Bacterial Vaccine 192 
Therapeutic Vaccination 192 
Results • 193 
CHAPTER IX. 
The Microscopic and Cultural Study of Bacteria. 
Methods for the Microscopic Study of Bacteria 195 
I. Examination of Living Bacteria 196 
A. Hanging Drop 196 
B. Hanging Block 196 
C. Dark-field Illumination and Ultramicroscopic Examination . 197 
D. Intra- vitam Staining 198 
II. Staining of Bacteria 198 
A. Chemistry of Stains 198 
B. Preparation of Stains 198 
C. Technique of Staining Bacteria 198 
D. Intensive Stains for Bacteria 199 
E. Stains for Special Structures of the Bacterial Cell .... 199 
F. Differential Stains for Bacteria 202 
III. Staining Bacteria in Tissues 207 
IV. Methods and Media for Cultivation of Bacteria 208 
Preparation of Glassware 209 
Preparation of Meat Infusion 210 
The Reaction of Media 211 
The Clarification of Media 218 
The Distribution of Media 218 
Sterilization of Media 219 
Storage of Media 224 
The Preparation of Nutrient Bouillon (Broth) 224 
Eberson's Yeast Medium 228 
Loffler's Blood Serum 229 
Coagulated Serum 229 
Hiss Serum-water Media 229 
Endo Medium for the Isolation of Typhoid, Paratyphoid and 
Dysentery Bacilli 229 
Lactose Litmus Agar 231 
Blood-agar • 231 
Ascitic and Hydrocele Fhiid Media ". 231 
