METHODS FOR MICROSCOPIC STUDY OF BACTERIA 243 
2. Anaerobw Cultures in Fluid Medio.— A simple method of main- 
taining anaerobiosis in fluid media, sufficiently effective for ordinary 
Fig. 29. — Novy jar for anaerobic cultures. (Park.) 
usao-e, is to overlay a flask or test-tube containing glucose broth 
with a layer of albolene about 1 cm. in depth. Immediately before 
inoculation all residual oxygen in the medium should be removed 
Fi<;. 30. — Koch safety 
burner. (Park.) 
Fig. 31. — Dunham thermo- 
regulator. (Park.) 
Fig. 32. Roux Bimetallic 
regulator. (Park.) 
by an exposure of one-half hour in the Arnold sterilizer, or ten minutes 
in an autoclave. The liquid is cooled rapidly to minimize reabsorp- 
