PLATE IV 
Cultivation of Spirochaeta Refringens. (Noguchi.) 
Fig. 1.— a schematif drawing of Spiioehieta refriugen.s from pure culturo.s (dark field). 
Fig. 2. — Spirochaeta refringen.s from a three weeks' old pure culture in ascitic tissue agar, at 
87° C. (dark field). X 1100. 
Fig. 8. — Spirochaeta refringens from a pure culture seven days old (dark field). X 1100. 
Figs. 4 and 5. — Spirochaeta refringens from a pure culture two weeks old (dark field). X 1100. 
