FILTERABLE VIRUSES 
633 
The treatment is most satisfactory when the diagnosis is made in 
the preparalytic stage of the disease, and injections practised at that 
time. 
About 15 cc. of serum (fresh as possible, and passed through a 
Berkefeld filter to insure sterility) is injected after the removal of a 
slightly greater volume of spinal fluid. This is repeated every twenty- 
four hours for two or three days, or at intervals of twelve hours in 
the more severe cases. The results of this method of treatment are 
shown graphically in the following tabulations: 
No. of 
cases. 
Prophylactic treatment. 
Paralytic treatment. 
Treated with: 
No 
paral- 
ysis. 
Paralysis with 
final recovery. 
Died. 
Total. 
Recovery. 
Died. 
Com- 
plete. 
Partial. 
Com- 
plete. 
Partial. 
Immune serum 
Normal serum 
54 
10 
44 
9 
5 
5 

1 
119 
33 
8 
4 
66 
28 
45 
5 
Immunity.— One attack appears to confer immunity in man, but the 
evidence is not conclusive. Flexner and Lewis' have been unsuccessful 
in reinfecting monkeys which have recovered from a typical infection 
and they lean toward the view that one attack confers immunity in 
these animals. Flexner and Amoss,^ using a non-fatal strain of the 
virus, were also unsuccessful in reinfecting animals. Aycock and 
Kagan^ report promising results in immunizing monkeys with repeated 
intracutaneous injections of the Noguchi virus. 
The characteristic disease has not been produced in experimental 
animals other than primates. 
Considerable interest attaches to the pleomorphic coccus isolated 
by Rosenow,"* Mathers,^ and others, from the spinal fluid of cases of 
poliomyelitis, and demonstrated in smears and sections of the brain 
and cord of undoubted cases of the disease. Cultures injected into 
young rabbits and guinea-pigs localize almost specifically in the 
nervous system and induce these lesions, accompanied by paralyses, 
which are strikingly reminiscent of poliomyelitis in man. Culti- 
vations from such lesions were successful, and the organism has been 
shown to be filterable through suitable stone filters. Also, the organism 
is specifically agglutinated by sera of man and monkeys which had 
recovered from poliomjelitis, and injections of the coccus into experi- 
mental animals appear to render such animals refractory to intra- 
1 Loc. cit. 2 Jour. Exp. Med., 1924, 39, 191. 
3 Jour. Immunol., 1927, 14, 85. 
^ Rosenow and Wheeler : Jour. Infec. Dis., 1918, 22, 281. Rosenow and Gray: Ibid., 
p. .345. Rosenow, Towne and von Hess: Ibid., p. 313. Rosenow: Ibid., p. 379. 
^ Mathers and Weaver: Ibid., p. 559. 
