810 
Fishery Bulletin 95(4), 1997 
Appendix 
Appendix Table 1 
Allele frequencies for 11 allozyme loci tested in four populations of orange roughy. 
Locus 
Allele 
(rc=no. of 
fish) Ritchie 
Box 
Waitaki 
Puysegur 
Locus 
Allele 
(n=no. of 
fish) Ritchie 
Box 
Waitaki 
Puysegur 
Cck-1* 
1 
0.494 
0.500 
0.596 
0.551 
Idh-2* 
1 
0.417 
0.442 
0.180 
0.196 
2 
0.489 
0.500 
0.404 
0.444 
2 
0.583 
0.548 
0.809 
0.793 
3 
0.017 
0.000 
0.000 
0.005 
3 
0.000 
0.005 
0.011 
0.011 
n 
90 
81 
47 
99 
4 
0.000 
0.005 
0.000 
0.000 
Est-1* 
i 
0.051 
0.160 
0.093 
0.152 
n 
96 
94 
47 
94 
2 
0.222 
0.229 
0.372 
0.250 
Ldh-1* 
i 
1.000 
0.983 
0.979 
0.931 
3 
0.709 
0.606 
0.430 
0.585 
2 
0.000 
0.017 
0.021 
0.069 
4 
0.006 
0.005 
0.006 
0.013 
n 
96 
90 
47 
99 
5 
0.013 
0.000 
0.000 
0.000 
Ldh-2* 
i 
0.000 
0.000 
0.011 
0.000 
n 
79 
94 
43 
99 
2 
1.000 
0.980 
0.989 
0.980 
Gpi-1* 
i 
0.515 
0.551 
0.553 
0.517 
3 
0.000 
0.020 
0.000 
0.020 
2 
0.232 
0.253 
0.245 
0.265 
n 
96 
99 
47 
99 
3 
0.253 
0.191 
0.191 
0.214 
Mdh-1* 
i 
0.625 
0.729 
0.656 
0.745 
4 
0.000 
0.006 
0.001 
0.004 
2 
0.375 
0.271 
0.344 
0.250 
n 
99 
89 
47 
99 
3 
0.000 
0.000 
0.000 
0.005 
Gpi-2* 
i 
0.015 
0.033 
0.042 
0.058 
n 
96 
94 
48 
98 
2 
0.970 
0.944 
0.948 
0.860 
Mpi-1* 
i 
0.037 
0.027 
0.052 
0.057 
3 
0.015 
0.022 
0.010 
0.081 
2 
0.957 
0.968 
0.948 
0.943 
n 
99 
90 
48 
99 
3 
0.000 
0.005 
0.000 
0.000 
Idh-1* 
i 
0.030 
0.011 
0.106 
0.083 
4 
0.005 
0.000 
0.000 
0.000 
2 
0.970 
0.979 
0.883 
0.897 
n 
94 
94 
48 
97 
3 
0.000 
0.005 
0.011 
0.021 
Pgm-1* 
i 
0.116 
0.144 
0.117 
0.126 
4 
0.000 
0.005 
0.000 
0.000 
2 
0.879 
0.850 
0.883 
0.874 
n 
99 
94 
47 
99 
3 
0.005 
0.006 
0.000 
0.000 
n 
99 
90 
47 
99 
Appendix Table 2 
Numbers of composite mtDNA D-loop haplotypes observed 
in four populations of orange roughy. The composite 
haplotypes are based on the restriction enzymes BstU I, 
Cfo I, Msp I, and Nla III. 
Haplotype 
Ritchie 
Box 
Waitaki 
Puysegur 
AABA 
21 
19 
5 
14 
BBBA 
11 
18 
21 
7 
AC BA 
0 
0 
2 
0 
AAAA 
2 
0 
0 
0 
AABB 
1 
1 
1 
3 
AABC 
1 
0 
0 
0 
AACA 
1 
0 
0 
0 
ABBA 
4 
4 
6 
10 
BABA 
2 
6 
6 
6 
