34 
THIRTEENTH REPORT. 
tions were obtained after heating the cold room treated solutions to 
the extreme heat of 110°C for 20 min. 
Table No. 1 indicates some of the results repeatedly obtained as it 
includes a few parts of a variously controlled experiment. 
TABLE NO. 1.- — Exp. No. 3; Test No. 8; Sample B. 
• 
Tube. 
Treatment 
of solution. 
Heat temper- 
ature. 
Heat 
time. 
Digestion 
hours. 
24. 
48: 
5 A 
Fresh . . 
1 io°C 
20 Min . 
3 A 
24 hrs. 0°-S°C. 
110°C 
20 Min . . . 
+ 
1 A 
168 hrs. 0°-8°C 
110°C 
20 Min . . . 
4- 
6 A 
Fresh . . . 
+ 
+ 
Control. 
4 A 
24 hrs. 0°-8°C. 
+ 
-i- 
Control. 
2 A '. 
168 hrs. 0°-8°C 
+ 
Control. 
The importance of the cold and not the time element in the above 
protection is shown by tests such as are indicated by table No. 2. 
TABLE NO. 2— Exp. No. 15. Sample C. 
Tube. 
Treatment of 
solutions. 
Heating. 
Time. 
Digestion 
hours. 
8. 
24. 
4 days at 35 °C. . 
100 °C 
20 Min. . . 
4 days at 23 °C. . 
100°C 
20 Min. . . 
— 
— 
3 
4 days at 0°C. . . 
100 °C 
20 Min. . . 
- 1 - 
+ 
1 \ 
4 days at 35 °C. . 
4- 
Control. 
2 A 
4 days at 23 °C. . 
+ 
+ 
Control. 
3 \ 
4 days at 0°C . . . 
+ 
Control. 
Thus we see that the room temperature and above offer little resist- 
ance to the solutions, although certain results show that the room tem- 
perature protects more nearly than the warmer one. 
STERILE DIGESTION. 
In all the tests where ox-fibrin was used for the digestion the ques- 
tion of the possible bacterial action was never eliminated, although the 
tests were at all times comparable. Thus it was attempted to obtain 
digestions under sterile conditions with a solution that had been ster- 
ilized by heat. 
Table No. 3 shows some of the results where well washed sterile fibrin 
from rabbits blood was used to test the digestive power of solutions after 
various heatings. 
