206 
THIRTEENTH REPORT. 
V 
This suggestion was given further attention by P. Vuillemin who, in 
1903, published a paper entitled: ^Importance taxinomique de Pappa- 
reil zygospore des Mucorineen.” In this paper lie gives a new classifi- 
cation of the Mucorales, laying great stress upon the manner in which 
the zygospore is formed in the different genera and using the method 
of zygospore formation as a distinguishing character in determining 
genera and even families. 
In his new classification he makes a new sub-family, namely, Zvgor- 
hynchees. Under this is included Dichanphora and Zygorhynchus heter- 
ogamus, formerly Mucor heterogamus, and also a new species Zygor- 
bynchus Moelleri. It is especially with this last form that this paper is 
concerned. 
In 1902 Prof. A. Moeller of Eberswalde found a species of Zygor- 
hynchus very similar to Zygorhynchus heterogamus, but which differed 
from it in some important characteristics, namely in the shape of the 
sporangia spores, the columella, and in the size of the zygospores, as 
well as in few miner differences. The fungus was reported to the Soci- 
ety of Mycology of France, and it was decided that the differences deter- 
mined by Moeller were significant enough to warrant calling the form 
which he found a new species and accordingly it was called Zygor- 
hvnchus Molleri after its distinguished discoverer. 
It was while identifying the various kinds of soil fungi in soil samples 
taken in Ann Arbor that a specimen of Zygorhynchus Moelleri was found. 
Because of the peculiar formation of the zygospore, it immediately at- 
tracted attention and a study with regard to its technical description 
and drawings seen in the accompanying plates were made. 
The fungus was grown on a culture medium of the following compo- 
sition : 
Primary potassium orthophosphate, KH 2 P0 4 
Calcium nitrate Ca (N0 3 ) 2 
Magnesium sulphate MgS0 4 
Cane sugar C G H 22 0 5 
Agar 2 % or gelatin 30%. 
One litre of distilled water. 
mol. wt. 
100 
mol. wt. 
100 
mol. wt. 
1000 
mol. wt. 
100 
It was also grown on a very rich medium containing beef extract and 
gelatin. In both cases it formed immense numbers of zygospores with 
only a limited number of sporangia. The former medium seemed to ac- 
celerate fruitification and to inhibit to a certain extent the growth of 
the vegetative mycelium. This was probably due to the poor culture 
medium. At ordinary temperatures, 68° to 75° F., zygospores were 
formed in as short a period as three days from the time of inoculation. 
