MICHIGAN ACADEMY OF SCIENCE. 
] 29 
pool, and those varying markedly from the pool eliminated. A new pool 
should then be made from the others. 
Cautions . — Samples of the blood designed for the opsonic test should be 
used as early as possible — they lose their opsonic power markedly after two 
or three days. The washed blood-corpuscles should be made up fresh. 
The bacterial emulsions should be made up fresh each time required, but an 
emulsion of tubercle may be kept in stock and used as needed. Each bacterial 
emulsion must be standardized by being opsonized with normal serum. 
Optical tests for the density of an emulsion are of value in that the}' enable 
one to dilute an emulsion to approximately the correct amount, but should 
not be relied on to take the place of the normal phagocytosis. The centrifuge 
used in preparing the blood-corpuscles should run without vibration, other- 
wise the leucocytes are liable to be disintegrated. 
BIBLIOGRAPHY. 
(To Jan. 1st, 1908.) 
Aaron, Chas. D.: “A Clinician’s Observations of Opsonic Therapy,” New 
York Med. Jour., Dec. 1, 1906, vol. 84, p. 1069. 
Albert, H.: “The Opsonic Index and Vaccine Therapv,” West. M. Rev. 
(Omaha), 1907, xii, 332-335. Iowa M. J ., 1907, xiv, 68-75. 
Allen, Albert H.: ‘‘Homologous Bacteria as a Vaccine in Tuberculosis,” 
Jour. Amer. Med. Asso., July 20, 1907, vol. 49, p. 202. 
Allen, R. W. : “Muscle Plasma: its Opsonic Power and Function in Phag- 
ocytosis,” Brit. Med. Jour., Aug. 25, 1906, vol. 2, p. 427. 
Amberg, Samuel: “The Opsonic Content of the Blood of Infants,” Jour. 
Amer. Med. Asso., Jan. 26, 1907, vol. 48, p. 304. 
Arkman, J. : “An Aspect of Vaccines and Antitoxins,” Lancet (Bond.), 
1907, ii. 
Aspelin, E.: [Remarks on the So-called Opsonins in the Serum], Alim. 
Sven. Lakartidn , (Stockholm), 1905, vol. 2, p. 385. 
Axamit unci Tsuda: “Versuche uber die Spezifitat cler Opsonischen Wir- 
kung des Normalserums,” Wiener klinische Wochenschrift, Aug. 29, 1907, 
No. 35, p. 1045. 
Bacher, St. : “Uber Beeinflussung der Phagocytose durch Normales Serum,” 
Zeitschrift fur Hygiene und Infektionskrankh., 1907, vol. 56, p. 33. 
Balban, W. : “Die Opsonin-lehre in Theorie und Praxis,” Wien. med. 
Presse , 1907, xliii, 1281-1289. 
Baldwin and Kinghorn: “Studies on Immunity in Tuberculosis: the 
Properties of the Serum of Immunized Rabbits,” Med. Neivs, Sept. 30, 1905, 
vol. 87, p. 636. 
Bancli, J.: “Contributo alio studio dell endotossina del bacillo di Loffler— 
Indice Opsonico dei seri antidifterici,” Centralblatt fur Bakt., Sept. 19, 1907, 
Part 1, Referate, vol. 40, Nos. 5 and 6, p. 169. 
Bandi, J.: “Agressina del Bacillo difterico: specificita ed azione antiop- 
sonica di essa,” Centralbl. fur Bakt., Sept. 19, 1907, Part 1, Referate, vol. 
40, Nos. 5 and 6, p. 170. 
Banks, A. G. : “The Variations in Scarlet Fever of the Opsonic Power of 
Streptococci,” Jour, of Path., Oct., 1907, vol. 12, No. 1, p. 113. 
Barr, Bell and Douglas: “A Case of Infective Endocarditis Cured by the 
Inoculation of a Vaccine Prepared from Organisms Found in the Patient’s 
Blood,” Lancet, Feb. 23, 1907, vol. 1. p. 499. 
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