464 INOCULATION FOR PLEURO-PNEUMONIA IN CATTLE. 
10 th. — The third inoculated wounds are inflamed. The 
swelling and soreness are quite equal with that of the second 
at the same distance of time from the inoculation. 
1 <lbh. — The wounds have a more unhealthy condition than 
any of the preceding. The lips are gaping, and the purulent 
discharge is thin and discoloured. 
14^. — Slight ulceration is going on. 
\&th. — The incisions are more healthy, and time alone is 
required to effect their healing ; they will therefore not again 
be reported. 
Still making these three cows the subjects of experiment, 
we determined in the next place to inoculate them again with 
fluid from the lung and deep incisions ; this was done, and in each 
case the wounds comported themselves as before . 
The sero-purulent discharge was taken as early as possible 
from these cc lung-inoculated ” places and used on other cows , in 
a like manner as it had been in the former experiments on the 
animals themselves : a similar result attended this procedure. 
Two sheep were also inoculated with the same purulent fluid 
at the same time, and the wounds in these animals inflamed 
and suppurated as in the cows. 
We shall now return to the two cows of which no mention 
has been made since January 20th, when they were for the 
last time ^successfully inoculated by superficially puncturing 
the skin. On February 1st these animals were inoculated by 
a deep and roughly-made puncture, after the plan we had wit- 
nessed in Belgium. At the end of a few days their tails were 
swollen and tender on the application of pressure. The in- 
flammation increased, and by the eighth day of inoculation 
pus was being discharged from the incisions. A small ulcer 
formed near to the place of inoculation in one case, and delayed 
the healing process. In the other case no such event occur- 
red. By the l6th the wounds were cicatrising. 
These being what are described as successful inoculations, 
we were anxious to inoculate these animals again in the same 
manner, to ascertain their capability of taking a second time. 
This was done. In one cow the effects were as marked as 
before, in the other the inoculation failed. 
The animals were next inoculated with purulent fluid taken 
from an inoculated sore, the incision being made on one side 
of the labia , while on the other side a similar incision, charged 
with an irritating medicinal agent , was also made, that the 
effects might be contrasted. The wound in which the pus 
was placed became inflamed the soonest and to the greatest 
extent, but so slight was the difference between them, that no 
person ignorant of the operation would have noted it, or any 
other peculiarity. 
