580 INOCULATION FOR PLEURO-PNEUMONIA. 
but all those which had been inoculated have remained free 
from it up to this very day. Afresh inoculation was practised 
on thirty beasts the 26th of December last, who had -not 
been so previously. Of this experiment, I shall recount the 
issue. 
3. Encouraged by success, I anxiously looked for fresh 
opportunity of continuing my experiments. I did not wait 
long. On the 21st of last July, M. Benoist, mayor of 
Marienat, and M. Benoid Camille, placed at my disposal 
their fine dairy of the Roche domain, where pleuro-pneumonia 
had already attacked fourteen beasts. Inoculation was practised 
on 142 beasts, at the origin and extremity of the tail, by means 
of five or six punctures, in order to introduce the largest 
quantity possible of the virus, without being previously en- 
gaged with consecutive accidents, such as M. Willems had 
witnessed. From the fifteenth to the twentieth day, there 
arose on the inoculated part considerable tumefaction, causing 
the loss of the tail to two cows, and destroying two others 
in whom this tumour extended to the vulva, anus, and mus- 
cles of the croup, as far as the pelvic cavity. Apart from 
these light accidents, and with regard to the number of 
animals operated on, no beast after this period has presented 
the slightest symptom of pleuro-pneumonia. 
4. On the 23d of the same month, I inoculated 25 beasts 
at M. Fabre’s house ; there the same operation was attended 
with similar success, with the exception of a single bull, who 
died in the same manner as the cows at Roche. So that 
167 beasts, inoculated in the same week, have been for 
upwards of six months preserved from every taint of the 
epizootic. The three notable losses would certainly have 
been prevented if the operation had been confined to the 
lower part of the tail. The brilliant results obtained at the 
houses of these two proprietors have made a great sensation 
in the country, and justly popularised a remedy against 
which had arisen some days before so many prejudices. 
5. Gn the 20th of Oct., I inoculated, at M. Chavaroche’s 
house, 36 animals, and from that time I have heard no more 
of them. 
6. On the 22d of the same month, I operated on 74 beasts 
belonging to M. Maillance, with the same success. 
7. On the 11th of November, inoculation was performed 
on 56 beasts on the domain of Ambesse, belonging to 
Capt. Fonteille. 
8. On the 14th of the same month, the same operation 
was performed on seventy-two animals on the domain of 
Landel. 
