418 
Fishery Bulletin 99(3) 
Acknowledgments 
I thank Belinda Curley and Ben Stewart for their assis- 
tance with collection of fish, Marti Jane Anderson for 
discussions regarding statistical analyses, and Robert 
McQuilty (RPA hospital) for use of the ICP-MS. I also 
thank Marti Jane Anderson and Sophie Dove for useful 
comments on the manuscript. The study was conducted 
while BMG held an ARC postdoctoral fellowship and was 
funded by an ARC grant and research funding from the 
University of Sydney. 
Literature cited 
Bagenal, T. B., F. J. H. Mackereth, and J. Heron. 
1973. The distinction between brown trout and sea trout 
by the strontium content of their scales. J. Fish Biol. 
5:555-557. 
Beauchemin, D., J. W. McLaren, S. N. Willie, and S. S. Berman. 
1988. Determination of trace metals in marine biological 
reference materials by inductively coupled plasma mass 
spectrometry. Anal. Chem. 60:687-691. 
Behrens Yamada, S., T. J. Mulligan, and D. Fournier. 
1987. Role of environment and stock on the elemental com- 
position of sockeye salmon ( Oncorhynchus nerka) verte- 
brae. Can. J. Fish. Aquat. Sci. 44:1206-1212. 
Belanger, S. E., D. S. Cherry, J. J. Ney, and D. K. Whitehurst. 
1987. Differentiation of freshwater versus saltwater striped 
bass by elemental scale analysis. Trans. Am. Fish. Soc. 
116:594-600. 
Campana, S. E., A. J. Fowler, and C. M. Jones. 
1994. Otolith elemental fingerprinting for stock discrimina- 
tion of Atlantic cod ( Gadus morhua ) using laser ablation 
ICPMS. Can. J. Fish. Aquat. Sci. 51:1942-1950. 
Campana, S. E., and J. A. Gagne. 
1995. Cod stock discrimination using ICPMS elemental 
assays of otoliths. In Recent developments in fish otolith 
research (D. H. Secor, J. M. Dean, and S. E. Campana, eds.), 
p. 671-691. Univ. South Carolina Press, Columbia, SC. 
Campana, S. E., J. A. Gagne, and J. W. McLaren. 
1995. Elemental fingerprinting of fish otoliths using ID- 
ICPMS. Mar. Ecol. Prog. Ser. 122:115-120. 
Campana, S. E., and J. D. Neilson. 
1985. Microstructure of fish otoliths. Can. J. Fish. Aquat. 
Sci. 42:1014-1032. 
Clarke, K. R., and M. Ainsworth. 
1993. A method of linking multivariate community struc- 
ture to environmental variables. Mar. Ecol. Prog. Ser. 92: 
205-219. 
Clarke, K.R., and R.M. Warwick. 
1994. Change in marine communities: an approach to statis- 
tical analysis and interpretation. Natural Environment 
Research Council, UK, 144 p. 
Coutant, C. C., and C. H. Chen. 
1993. Strontium microstructure in scales of freshwater and 
estuarine striped bass ( Morone saxatilis ) detected by laser 
ablation mass spectrometry. Can. J. Fish. Aquat. Sci. 50: 
1318-1323. 
Date, A. R. 
1991. Inductively coupled plasma mass spectrometry. Spec- 
trochimica Acta Review 14:3-32. 
Degens, E. T., W. B. Deuser, and R. L. Haedrich. 
1969. Molecular structure and composition of fish otoliths. 
Mar. Biol. 2:105-113. 
Dove, S. G. 
1997. The incorporation of trace metals into the eye lenses 
and otoliths of fish. Ph.D. diss., Univ. Sydney, Sydney, 
201 p. 
Dove, S. G., B. M. Gillanders, and M. J. Kingsford. 
1996. An investigation of chronological differences in the 
deposition of trace metals in the otoliths of two temperate 
reef fishes. J. Exp. Mar. Biol. Ecol. 205:15-33. 
Dove, S. G., and M. J. Kingsford. 
1998. Use of otoliths and eye lenses for measuring trace- 
metal incorporation in fishes: a biogeographic study. Mar. 
Biol. 130:377-387. 
Edmonds, J. S., N. Caputi, and M. Morita. 
1991. Stock discrimination by trace-element analysis of oto- 
liths of orange roughy (Hoplostethus atlanticus), a deep- 
water marine teleost. Aust. J. Mar. Freshwater Res. 42: 
383-389. 
Edmonds, J. S., R. C. J. Lenanton, N. Caputi, and M. Morita. 
1992. Trace elements in the otoliths of yellow-eye mullet 
( Aldrichetta forsteri) as an aid to stock identification. Fish. 
Res. 13:39-51. 
Edmonds, J. S., M. J. Moran, and N. Caputi. 
1989. Trace element analysis of fish sagittae as an aid to 
stock identification: pink snapper ( Chrysophrys auratus ) 
in Western Australian waters. Can. J. Fish. Aquat. Sci. 
46:50-54. 
Fowler, A. J., S. E. Campana, C. M. Jones, and S. R. Thorrold. 
1995. Experimental assessment of the effect of tempera- 
ture and salinity on elemental composition of otoliths 
using laser ablation ICPMS. Can. J. Fish. Aquat. Sci. 52: 
1431-1441. 
Gallahar, N. K., and M. J. Kingsford. 
1996. Factors influencing Sr/Ca ratios in otoliths of Girella 
elevata: an experimental investigation. J. Fish Biol. 48: 
174-186. 
Gauldie, R. W., and D. G. A. Nelson. 
1990. Otolith growth in fishes. Comp. Biochem. Physiol. 
97A:119-135. 
Gillanders, B. M., D. J. Ferrell, and N. L. Andrew. 
1997. Determination of ageing in kingfish (Seriola lalandi) 
in New South Wales. Fisheries Research Development 
Corporation (ISBN 0 7310 9406 9), 103 p. 
Gillanders, B. M., and M. J. Kingsford. 
1996. Elements in otoliths may elucidate the contribution 
of estuarine recruitment to sustaining coastal reef pop- 
ulations of a temperate reef fish. Mar. Ecol. Prog. Ser. 
141:13-20. 
Gunn, J. S., I. R. Harrowfield, C. H. Proctor, and R. E. Thresher. 
1992. Electron probe microanalysis of fish otoliths — evalu- 
ation of techniques for studying age and stock discrimina- 
tion. J. Exp. Mar. Biol. Ecol. 158:1-36. 
Hamada, M., T. Nagai, N. Kai, Y. Tanoue, H. Mae, M. Hashimoto, 
K. Miyoshi, H. Kumagai, and K. Saeki. 
1995. Inorganic constituents of bone of fish. Fish. Sci. 61: 
517-520. 
Hartl, D. L., and A. G. Clark.. 
1989. Principles of population genetics. Sinauer Associ- 
ates Inc, Sunderland, MA, 682 p. 
Hellou, J., W. G. Warren, J. F. Payne, S. Belkhode, and P. Lobel. 
1992. Heavy metals and other elements in three tissues of 
cod, Gadus morhua from the Northwest Atlantic. Mar. 
Poll. Bull. 24:452-458. 
Hill, K. T., G. M. Cailliet, and R. L. Radtke. 
1989. A comparative analysis of growth zones in four calci- 
fied structures of Pacific blue marlin, Makaira nigricans. 
Fish. Bull. 87:829-843. 
