530 
Fishery Bulletin 99(4) 
Table 1 
Primers for PCR amplification of coho salmon mitochondrial DNA (mtDNA), location in relation to O. mykiss (Zardoya et al., 1995), 
PCR fragment sizes, and fragment overlaps (bp). 
0. mykiss 
Fragment 
Region 
Sequence 
locations 
size 
Overlap 
Source 
12S/16S 
5' AATTCAGCAGTGATAAACATT 3' 
1234-1254 
1 
5' AGATAGAAACTGACCTGGATT 3' 
3615-3635 
2402 
121 
1 
ND1/ND2 
5' ACCTCGATGTTGGATCAGG 3' 
3515-3533 
1 
5' ATTAAAGTGNTTGA(T/G)TTGCATTC 3' 
6181-6203 
2689 
-430 
1,5 
COI/COII 
5TAATCGTCACAGCCCATGCCTTCGT 3' 
6634-6658 
2 
5' GGTCAGTTTCAGGGTTCAGGTTTAGC 3' 
9079-9104 
2471 
166 
2 
A8/COIII 
5' CTAGTGACATGCCCCAACTCAACC 3' 
8939-8962 
2,3 
5' TCATAAGGCGGTCATGGACTTAAACC 3' 
11028-11053 
2115 
480 
2,3 
ND3/ND4 
5' TTAC GCGTAT AAGTGACTTC C AA 3' 
10574-10596 
2,3 
5' TTTTGGTTCCTAAGACCAATGGAT 3' 
12881-12904 
2331 
32 
2,3 
ND5/ND6 
5' AAC AGCTC ATC C ATTGGTCTTAGG 3' 
12873-12896 
2, 4, 5 
5' TTAC AAC G ATGGTTTTTC ATGTC A 3' 
15319-15342 
2470 
19 
2, 4, 5 
Cytb/D-loop 
5' TGAA( G/A 1ACCACCGTTGTTATTC AA 3' 
15324-15347 
2, 4, 5 
5' TAGGGCCTCTCGTATAACCG 3' 
1321-1340 
2659 
107 
2, 4,5 
12S/16S 
1 Consensus from Anderson et al. (1981); Anderson et al. (1982); Bibb et al. (1981); Roe et al. (1985); Chang et al. (1994). 
2 Unpublished mtDNA sequence data from our lab. 
3 Thomas and Beckenbach (1989). 
4 Cronin et al. (1993). 
5 Carney et al. (1997). 
