134 
BULLETIN OF THE BUREAU OF FISHERIES 
" The experimental specimens were tested for a short time in water alone before 
the liquor was run in. When the experimental fluid started this was allowed to 
run into the chamber already full of pure water, which it slowly replaced. 
The kymograph records were fixed in shellac, and then marked off into hourly 
periods for recording the results. At times one of the levers would not be close 
enough to the paper to write the record, and several hours of records might thus 
be lost. In such a case the number of hours per day that the specimen was open 
was computed for the period of existing records and then calculated on the basis of 
the 24-hour period. 
EXPERIMENTS WITH ACID LIQUOR 
Experiment No. 1 (6 parts per thousand, October 15 to 19, 1929 ): — One specimen was 
in each of the three experimental tanks. The first and third were controls and the 
Figure 6.— Graph showing the number of Figure 7.— Graph showing the cessation of activity of the liquor-treated specimen in 
hours per day which each specimen em- experiment No. 1. The number of closing movements is plotted for each hour from 
ployed in experiment No. 1 was open. beginning of gaping of the shells until movement had ceased 
One specimen was subjected to sulphite 
liquor solution (6 parts per 1,000), while 
two were controls in pure water 
second, experimental. All specimens were in pure, running sea water on October 15 
and up until 7.50 p. m. on the 16th, when liquor was started dripping into the mixing 
chamber which supplied the experimental oyster. In this case water was flowing 
at the rate of 134 cubic centimeters per minute and pure liquor was entering at 
0.8 cubic centimeters per minute making a final solution of 6 parts per thousand. 
The pH of the solution in the experimental tank was between 5.8 and 6.1, while that 
of the control water was 7.7. During the test the temperature of the water in all 
tanks fluctuated between 10° and 14° C. 
In Figure 6 are shown graphs of the number of hours per day that each specimen 
remained open. There is little difference between the specimens in this respect, 
but while the two controls remained open and highly active, the experimental specimen 
