138 
BULLETIN OF THE BUREAU OF FISHERIES 
sea water. The pH of the solution varied from 6.5 to 7.0. Liquor was started at 
3.50 p. m. February 3, and continued until the end of the experiment. 
The records (fig. 13) show the time of death of the two specimens, as well as the 
number of hours per day that they remained open. Specimen No. 5, during the 
period from February 4 until just before it started gaping wide on the 14th, was open 
an average of 16.62 hours per day and was dead on the 16th, 12 days after the liquor 
was started. On the other hand, specimen No. 6 averaged 13.32 hours per day 
open from the 4th until the 24th, 
after which death occurred follow- 
ing 21 days of treatment. 
Experiments Nos. 12 and 13 
( January 3 to January 10, 1930 ). — 
This is an incomplete series due 
to the fact that the water system 
was frozen on January 10 and 
experiments had to bel stopped. 
However, it shows somejfeffect of 
the liquor in the short time that 
the test was continued. The 
water was heated and the tempera- 
ture varied between 14° and 18° C. The stock liquor solution consisted of 1 part 
pure liquor to 19 parts sea water. 
Experiment No. 13 ( 3.8 parts per thousand) . —The rate of flow of water was 61 
cubic centimeters per minute and that of liquor solution 4.66 cubic centimenters per 
minute, producing a concentration of about 3.8 parts pure liquor per thousand parts 
water. The pH varied be 
FEBRUARY 
Figure 13. — Graph showing the number of hours per day which the 
specimens in experiment No. 18 (3.2 parts per 1,000) were open. 
Compare with the controls (fig. 12) 
f— g Ow 
y _ 
Z 
1 g o SPECIMEN "1 1 
- g-g SPECIMEN *3 - 
1 o-G SPECIMEN *5 
j x~x SPECIMEN *2 
j x-x SPECIMEN *4 ^ 
^ x-x SPECIMEN *6 
3 4 5 6 7 8 9 
10 
3 4 5 6 7 8 9 10 
3456789 10 
JANUARY 
JANUARY 
JANUARY 
EXPERIMENT 13 
EXPERIMENT 12 AND 13 
tween 6.6 and 7.0. 
From January 3 until 
the 7th at 4.30 p. m., when 
liquor was started, the spec- 
imens were in pure, running 
water, and both control and 
experimental oysters (fig. 
14) were open a large part of 
the time. After the liquor 
was started both experi- 
mental specimens showed 
an immediate reaction. 
During the three days from 
the 8th to the 10th, specimen No. 1 averaged 11.46 hours per day open, while specimen 
No. 2 averaged 18.96 hours per day open. At the same time the control specimens 
Nos. 3 and 4 were open, respectively, 23.73 and 23.5 hours per day. This is too short 
a treatment for these averages to be of much significance, but the appearance of the 
records showing the immediate effect of the liquor on specimens Nos. 1 and 2 is 
important. 
Experiment No. 12 ( 1.8 parts per thousand).— Water flowed at 116 cubic centi- 
meters per minute and liquor solution (1: 19) at 4.25 cubic centimeters per minute, 
producing the above-stated concentration in parts of pure liquor per thousand. 
The pH was usually between 6,8 and 7.1. This concentration is lower than that 
Figure 14.— Graphs showing the reactions of the specimens in experiments Nos. 12 
(1.8 parts per 1,000) and 13 (3.8 parts per 1,000) and controls 
