Gharrett et al.: Identification of Sebastes spp. by restriction site analysis 
57 
S. ciliatus on the tree (not shown). Haplotypes of S. aleu- 
tianus and S. borealis (subgenus Zalopyr) were found in 
the same general region of the tree, but are not sister 
taxa. Likewise, the two representatives of Acutomentum, 
S. alutus and S. brevispinis, were not monophyletic sister 
taxa. Disparities, such as we observed between relation- 
ships of haplotype and assignments of subgenera, have 
also been reported for allozyme comparisons (Seeb, 1986) 
and mtDNA cytochrome b sequences (Johns and Avise, 
1998; Rocha-Olivares, 1998a; Rocha-Olivares et al., 1999a, 
1999b). The members of subgenera Acutomentum and Al- 
losebastes, in particular, seem discordant with trees. It is 
important to recall that the systematics is not unequivo- 
cal and controversies date back more than a century (e.g. 
Cramer, 1895). Therefore, discrepancies between the mo- 
lecular-based comparisons and current systematic place- 
Maximum likelihood 
Maximum parsimony 
L S. alutus A Acutomentum 
S. alutus B Acutomentum 
S ciliatus Sebastosomus 
S aleutianus A Zalopyr 
, S. aleutianus B Zalopyr 
p* S ruberrimus A Sebastopyr 
S ruberrimus B Sebastopyr 
S borealis Zalopyr 
S brevispinis Acutomentum 
S . proriger Allosebastes 
S. zacentrus A Allosebastes 
S zacentrus D Allosebastes 
S zacentrus B Allosebastes —1 so 
S. zacentrus C Allosebastes 1 1 
S. variegatus Allosebastes , 
S. helvomaculatus A Sebastomus , 
S helvomaculatus B Sebastomus Jj°l 
90 . 
S babcocki A Rosicola 
S. babcocki B Rosicola 
S. melanops A Sebastosomus 
S. melanops B Sebastosomus 
S flavidus Sebastosomus 
S. maliger Pteropodus 
S. caurinus A Pteropodus 
S caurinus B Pteropodus 
H. hilgendorfi 
Sebastolobus alascanus A 
Sebastolobus alascanus B 
Sebastolobus alascanus D 
Sebastolobus alascanus C 
_L 
0.10 0.05 0 
Nucleotide substitutions 
Figure 1 
Maximum likelihood (PHYLIP; Felsenstein 3 ) and maximum parsimony consensus trees (PAUP ; Swofford, 
1998) trees estimated from restriction site data for haplotypes of 15 Sebastes spp., Helicolenus hilgendorfi , and 
Sebastolobus alascanus. Haplotype and restriction site data are provided in Appendix 1 and Table 3, respec- 
tively. On the maximum likelihood tree, and indicate distances that are significantly positive (P<0.05 and 
P<0.01, respectively). On the maximum parsimony consensus tree, the numbers at nodes indicate the percent- 
age of trees that included those branches. 
