Gharrett et al.: Identification of Sebastes spp by restriction site analysis 
61 
Appendix 1 
Restriction site locations for Sebastes spp., Helicolenus hilgendorfi, and Sebastolobus alascanus in the 12S/16S and ND3/ND4 mtDNA 
regions. The Sebastes species are listed in Table 2; five individuals of each species are represented. Sites were mapped by double 
digests. Haplotypes for each restriction endonuclease are presented for each mtDNA region. X’s denote presence and O’s denote 
absence of a site. (X)’s are sites that occur in the primers and were present in all PCR products. They were not used in the analysis. 
12S/16S 
Msp I 
Cfo I 
Rsa I hapotypes 
Mbo I haplotypes 
haplotypes 
Dde I haplotypes 
haplotypes 
sites 
A 
B 
c 
D 
E 
sites 
A 
B C 
D 
E 
F 
sites 
A 
B sites 
A 
B 
C 
D 
E 
F 
sites 
A 
B 
293 
O 
O 
o 
0 
X 
201 
O 
X X 
O 
o 
0 
65 
X 
X 
44 
X 
X 
X 
X 
X 
X 
537 
X 
X 
507 
O 
O 
X 
X 
o 
849 
X 
X X 
X 
o 
X 
766 
X 
X 
55 
X 
X 
X 
X 
X 
X 
602' 
X 
O 
588 
O 
O 
0 
0 
X 
1015 
X 
X X 
X 
X 
0 
1259 
O 
X 
976 
X 
X 
X 
X 
X 
0 
665' 
X 
X 
761 
X 
X 
X 
X 
X 
1403 
X 
X X 
X 
X 
X 
1390 
X 
X 
1043 
X 
X 
O 
X 
X 
o 
1898 
X 
X 
950 
o 
O 
o 
o 
X 
1507 
X 
O X 
X 
X 
X 
1535 
X 
X 
1056 
X 
X 
0 
X 
0 
0 
2268 
X 
X 
1000 
X 
X 
X 
X 
0 
1746 
O 
O O 
X 
0 
0 
2226 
X 
X 
1304 
X 
X 
X 
X 
X 
0 
1071 
X 
X 
X 
X 
0 
1984 
X 
X X 
X 
X 
X 
2403 
X 
X 
1735 
o 
X 
X 
X 
X 
0 
1263 
o 
0 
0 
0 
X 
2059 
X 
X X 
X 
X 
X 
2181 
X 
O 
X 
X 
X 
0 
1308 
0 
X 
0 
X 
X 
2228 
X 
X X 
X 
X 
X 
2393 
X 
X 
X 
X 
X 
X 
1358 
X 
X 
X 
X 
X 
2318 
X 
X X 
X 
X 
X 
2164 
0 
o 
0 
o 
X 
2388 
X 
X X 
X 
X 
X 
Sty I 
BstC I 
Hin 
fl haplotypes 
Hin 
d II haplotypes 
haplotypes 
Bst N I haplotypes 
haplotypes 
sites 
A 
B 
c 
D 
sites 
A 
B 
sites 
A 
B 
sites 
A 
B 
c 
D 
sites 
A 
B 
982 
O 
O 
X 
0 
1717 
X 
O 
295 
X 
O 
326 
X 
X 
X 
X 
87 
X 
X 
1291 
X 
X 
X 
o 
2053 
O 
X 
988 
O 
o 
0 
X 
600' 
X 
O 
2094 
0 
X 
X 
o 
2254 
X 
X 
1687 
O 
o 
X 
0 
664' 
X 
X 
1741 
O 
X 
0 
0 
729 
X 
X 
2416 
(X) 
(X) 
(X) 
X) 
1872 
X 
X 
2128 
X 
X 
ND3/ND4 
Cfo I haplotypes 
Bst U I haplotypes 
Hin 
fl haplotypes 
sites 
A 
B 
c 
D 
E 
F 
sites 
A B 
c 
D 
E 
F 
G 
sites 
A 
B 
C 
D 
E 
F 
G 
H 
709 
X 
O 
X 
X 
X 
X 
4 
(X) (X) 
(X) 
(X) 
(X) 
(X) 
(X) 
130 
O 
X 
O 
O 
o 
X 
O 
O 
1221 
o 
O 
o 
o 
X 
X 
344 
O 0 
0 
o 
0 
X 
o 
389 
O 
X 
X 
O 
0 
0 
o 
X 
1436 
o 
O 
0 
o 
X 
O 
1499 
O 0 
0 
0 
X 
o 
0 
494 
X 
X 
X 
X 
X 
X 
X 
X 
1741 
0 
X 
X 
X 
0 
O 
1854 
O 0 
o 
X 
o 
0 
0 
853 
O 
O 
O 
O 
0 
0 
o 
X 
1813 
o 
O 
X 
0 
0 
O 
2025 
O X 
X 
0 
o 
0 
0 
1448 
O 
O 
O 
O 
0 
0 
0 
X 
2306 
O X 
0 
0 
X 
X 
X 
1537 
O 
O 
O 
0 
o 
0 
X 
O 
1755 
O 
O 
O 
0 
X 
X 
0 
O 
1888 
O 
O 
O 
X 
0 
0 
0 
X 
2232 
X 
X 
X 
X 
X 
X 
X 
X 
Sty I haplotypes 
Msp I haplotypes 
Rsa I haplotypes 
sites 
A 
B 
c 
D 
E 
F 
sites 
A B 
c 
D 
E 
F 
G 
sites 
A 
B 
C 
D 
E 
F 
G 
H 
49 
X 
X 
X 
X 
X 
X 
30 
X X 
X 
X 
X 
X 
X 
346 
O 
O 
O 
X 
X 
O 
X 
X 
194 
O 
O 
o 
X 
X 
X 
933 
O O 
0 
0 
o 
X 
O 
742 
O 
O 
O 
O 
0 
0 
O 
X 
365 
X 
X 
X 
X 
0 
O 
1199 
O O 
0 
0 
o 
X 
O 
1077 
O 
O 
O 
O 
0 
X 
0 
O 
391 
O 
O 
0 
0 
X 
X 
1261 
O O 
0 
o 
0 
O 
X 
1231 
O 
O 
O 
o 
0 
0 
X 
O 
534 
X 
O 
X 
X 
X 
X 
1738 
X X 
X 
X 
X 
X 
X 
1492 
O 
O 
O 
0 
0 
0 
0 
X 
1258 
0 
0 
o 
o 
0 
X 
1826 
X X 
0 
X 
X 
0 
0 
1863 
O 
O 
X 
0 
X 
0 
0 
X 
1570 
o 
X 
X 
X 
X 
X 
1844 
O O 
o 
X 
X 
o 
o 
1985 
O 
X 
X 
X 
X 
X 
X 
X 
2311 
X 
X 
X 
X 
X 
X 
2073 
0 o 
0 
0 
o 
X 
o 
2110 
O X 
X 
0 
X 
o 
o 
continued 
