Fishery Bulletin 99(1) 
100 
Table 1 (continued) 
Ots 102 (continued) 
Alleles 
Harrison 
302 
Chilliwack 
White 
180 
LFr 
482 
Quesnel 
174 
Stuart 
255 
Nechako 
129 
Chilko 
114 
Bridge 
47 
Cottonwood 
48 
Mid Fr 
767 
Tete 
Jaune 
262 
Chilliwack 
Red 
29 
236 
0.043 
0.067 
0.052 
0.083 
0.045 
0.085 
0.053 
0.032 
0.021 
0.059 
0.053 
0.034 
241 
0.065 
0.05 
0.059 
0.055 
0.045 
0.043 
0.022 
0.085 
0.031 
0.045 
0.132 
0.069 
246 
0.05 
0.047 
0.049 
0.009 
0.024 
0.016 
0.031 
0.053 
0 
0.02 
0.074 
0.017 
251 
0.03 
0.042 
0.034 
0.026 
0.022 
0.019 
0.009 
0 
0.01 
0.018 
0.048 
0.034 
256 
0.038 
0.022 
0.032 
0.032 
0.012 
0.008 
0.044 
0.011 
0.073 
0.024 
0.122 
0.034 
261 
0.028 
0.039 
0.032 
0.052 
0.022 
0.023 
0.022 
0.032 
0.063 
0.032 
0.021 
0 
267 
0.022 
0.017 
0.02 
0 
0.029 
0.016 
0.009 
0 
0 
0.014 
0 
0.034 
273 
0.015 
0.003 
0.01 
0.009 
0.016 
0.008 
0.009 
0 
0 
0.01 
0 
0 
279 
0.005 
0.003 
0.004 
0.011 
0.002 
0 
0 
0 
0.021 
0.005 
0 
0 
285 
0.01 
0.014 
0.011 
0.006 
0 
0.004 
0 
0 
0 
0.002 
0 
0 
291 
0.013 
0 
0.008 
0 
0 
0.004 
0.013 
0 
0 
0.003 
0 
0.034 
297 
0.01 
0.014 
0.011 
0.009 
0 
0.004 
0 
0 
0 
0.003 
0.004 
0 
303 
0.003 
0 
0.002 
0.029 
0.004 
0.004 
0.004 
0.021 
0 
0.01 
0.004 
0 
309 
0.002 
0.006 
0.003 
0 
0 
0 
0 
0 
0 
0 
0 
0 
325 
0.005 
0 
0.003 
0.003 
0 
0 
0 
0 
0 
0.001 
0 
0 
321 
0.002 
0 
0.001 
0 
0 
0 
0 
0 
0 
0 
0 
0 
333 
0.002 
0 
0.001 
0 
0 
0 
0 
0 
0 
0 
0 
0 
Hobs 
0.714* 
0.628* 
0.699 
0.632* 
0.761* 
0.713* 
0.684* 
0.723 
0.833 
0.714 
0.775* 
0.862 
Hexp 
0.927 
0.928 
0.927 
0.931 
0.894 
0.93 
0.842 
0.894 
0.833 
0.906 
0.859 
0.897 
Table 2 
Primer sequences, PCR cycle, and gel-running conditions for microsatellite loci OtslOO, OfslOl, and Otsl02. 
Gel conditions 
Locus 
Primer sequence 
PCR cycle 
denature 
Anneal 
Extend 
No. of 
cycles 
% 
acrylamide 
Voltage 
(V) 
Time 
(h) 
Ots 100 
F 5TGAACATGAGCTGTGTGAG-3' 
R 5'-ACGGACGTGCCAGTGAG-3'. 
94°C/20s 
57°C/20s 
72°C/20s 
30 
7 
60 
18 
OfslOl 
F 5'ACGT CT G ACTT CAAT GAT GTTT-3' 
R 5'TATTAATTAT CCT CCAACCCAG-3' 
94°C/30s 
53°C/30s 
72°C/30s 
30 
8 
70 
17 
Ots 102 
F 5'AGG AT CCAATAAGG AGT G ATA-3' 
R 5ACTAGGTATCCCCTTAACCA-3' 
94°C/20s 
50°C/10s 
72°C/20s 
30 
6 
60 
17 
Data analysis 
A pedigree analysis was performed on chinook salmon 
families to document the inheritance of microsatellite 
alleles at each locus. Chinook salmon families were obtained 
from domesticated strains originating in Robertson Creek 
and Big Qualicum River, B.C. For each locus, we examined 
inheritance in 6 families (12 offspring per family). Popu- 
lation genetic data were analyzed by using GENEPOP 
version 3.1 (Raymond and Rousset, 1995a) and ARLEQUIN 
version 1.1 (Schneider et al., 1997). Allele frequencies and 
heterozygosities were estimated for each population at each 
locus and conformation to Hardy- Weinberg equilibrium 
(HWE) was tested with a simulated Fisher’s exact test (Guo 
and Thompson, 1992). A gametic disequilibrium test was 
also performed. Differences in allele frequencies among dif- 
ferent sample years, populations, and regions (populations 
grouped into regions) were examined by using pairwise 
tests in ARLEQUIN which is analogous to Fisher’s exact 
test (described in Raymond and Rousset, 1995b). An analy- 
sis of molecular variation (AM OVA) was also performed by 
using ARLEQUIN to measure the distribution of molecu- 
lar variance at several levels: among individuals, among 
samples taken in different years for the same population, 
among all populations (with year classes combined), and 
among regions. F-statistics (Wright, 1951) and their stan- 
