126 
Fishery Bulletin 99(1 ) 
Table t 
Loci analyzed in even-year pink salmon broodlines from Japan, Russia, and Alaska, their enzyme numbers and designations (Shak- 
lee et al., 1990), the tissue(s) and buffer(s) in which they were scored, and the level of variability observed. 
Enzyme 
Enzyme 
number 
Locus 
Tissue 7 
Buffer 2 
Level of 
variability 3 
Aconitate hydratase 
4.2. 1.3 
sAH* 
L 
8 
2 
mAH- 1 * 
H 
4,5,6 
2 
mAH-2 * 
H 
4,5,6 
3 
mAH-3* 4 ’ 5 
H,M 
4,5,6 
2 
mAH-4 * 4 ’ 5 
H,M 
4,5,6 
3 
Alanine aminotransferase 
2.6. 1.2 
ALAT* 4 
M 
2 
2 
Aspartate aminotransferase 
2.6. 1.1 
sAAT-1,2* 4 
M,H 
4,6 
2 
sAAT-3* 4 ’ 5 
E 
7 
3 
sAAT-4 * 
L 
8 
3 
mAAT-1* 4 
M,H 
4,6 
1 
mAAT-2 * 
L 
8 
1 
Creatine kinase 
2. 7.3.2 
CK-A1* 4 - 5 
M 
1 
2 
CK-A2* 45 
M 
1 
2 
CK-B 
E 
7 
2 
CK Cl* 
E 
7 
3 
CK-C2 * 
E 
7 
2 
Formaldehyde dehydrogenase 
1.2. 1.1 
FDHG* 4 
H 
2 
2 
Fumarate hydratase 
4.2. 1.2 
FH * 
M 
4 
3 
Glucose-6-phosphate isomerase 
5.3. 1.9 
GPI-A* 45 
M 
1 
2 
GPI-B1.2* 4 - 5 
M 
1 
2 
Glutathione reductase 
1.6. 4. 2 
GR* 4 
E 
4,5 
3 
Glycerol-3-phosphate dehydrogenase 
1.1. 1.8 
G3PDH-1 * 4 - 5 
M 
2,3 
3 
Guanine deaminase 
3. 5. 4. 3 
GDA* 
L 
7,8 
3 
L-Iditol dehydrogenase 
1.1.1.14 
IDDH 
L 
7 
2 
Isocitrate dehydrogenase 
1.1.1.42 
mIDHP- 1 4 
M,H 
4 
1 
mIDHP-2* 4 
M,H 
4 
1 
continued 
one collection were sAAT-3*, sAAT-4 *, mAH-2*, mAH-4*, 
CK-C1 * FH \ GDA *, G3PDH- 1 *, GR \ LDH-A 1 * sMDH-A 1 3 
sMDH-B2*, mMEP-1*, PEP LT \ PE PIG. PEPD-2*. and 
PGDH At 22 other variable loci, the frequency of the 
common allele was greater than 0.95 in all collections: 
sAAT 1.2 3 mAH-1 *, sAH*, mAH-3 *, ALAT\ CK-AP\ 
CK-A2*, CK-B * CK-C2*, FDHG*, GPI-A*, GPI-B2*, IDI)H \ 
mIDHP- 1 ' . LDH-BP3 LDH-B23 MPI . PE PI) -13 PGM 2*. 
TPI-2', and TPI-4*. The remaining 15 loci were monomor- 
phic for the same allele in all collections. 
The allelic frequencies observed are generally compara- 
ble to data for the few loci published by Russian geneti- 
cists (Altukhov et al., 1983; Salmenkova and Omelchenko, 
1983; Zhivotovsky et al., 1989; Kartavtsev, 1991; Kartavt- 
sev et al., 1992) except that we observed the PGDH 95 al- 
lele in all regions. Detection of that allele requires careful 
adjustment of the buffer pH, otherwise it migrates with 
the common allele. The pH at which Gharrett et al. ( 1988) 
analyzed PGDH did not distinguish that allele from the 
100 allele. 
Tests of conformance to Hardy- Weinberg expectations 
for genotypic frequencies were made for 126 locus-collec- 
tion combinations. Of these, four did not conform (P<0.05); 
this is fewer than would be expected at random. However, 
the sample size for most tests was small; therefore only 
large deviations from Hardy- Weinberg expectations would 
have been detected. Homogeneity within and among drain- 
ages, geographic regions, and continents or marine basins 
was tested by using 28 loci at which more than 5 variant 
alleles were observed (Table 2). Four rivers on Sakhalin Is- 
land were the only drainages from which multiple, tempo- 
rally stratified collections were sampled during the spawn- 
ing season. The numerous samples collected within the 
Ochepukha River system permitted treatment of its Zna- 
menka tributary as a fifth separate system. Only a subset 
of loci was scored in some collections, but at least one 
collection from each river was scored for the entire set 
of loci. No overall heterogeneity (P>0.05) was observed 
for any Sakhalin drainage, and only a few loci suggested 
heterogeneity (Table 2), which vanishes when corrections 
