Noll et al : Analysis of genetic structure of Asian and western Oncorhynchus gobuscha 
12 7 
Table 1 (continued) 
Enzyme 
Enzyme 
number 
Locus 
Tissue 7 
Buffer 2 
Level of 
variability 0 
Lactate dehydrogenase 
1.1.1.27 
LDH-A1* 45 
M 
1 
3 
LDH-A2* 4 - 5 
M 
1 
1 
LDH-B1* 4 
H 
1 
2 
LDH-B2* 
L 
1 
2 
LDH-C* 45 
E 
7 
1 
Malate dehydrogenase 
1.1.1.37 
sMDH-Al,2* 
L 
6 
3 
sMDH-Bl,2* 4 ’ 5 M 
2,3 
3 
Malic enzyme 
1.1.1.40 
mMEP-1* 4 ’ 5 
M 
3,4 
3 
Mannose-6-phosphate isomerase 
5.3.1.8 
MPI* 4 - 5 
H 
2 
2 
Peptidase: 
Cytosol non-specific dipeptidase (glycyl-leucine) 
3.4.-.- 
PEP A* 4 
M 
2 
1 
Tripeptide aminopeptidase (zinc enzyme) (leucyl-glycyl-glycine) 
3.4.-.- 
PEPB * 4 
M 
1 
3 
Leucyl-tyrosine peptidase 
3.4.-.- 
PEP-LT* 
M 
2,4 
3 
Phosphoglucomutase 
5. 4.2. 2 
PGM-2* 4 - 5 
M 
3 
2 
Phogluconate dehydrogenase 
1.1.1.44 
PGDH* 45 
E 
3 
3 
Phosphoglycerate kinase 
2. 7. 2. 3 
PGK-1* 
L 
8 
1 
PGK-2* 
L 
8 
1 
X-proline dipeptidase 
3.4.13.9 
PEPD-1* 45 
M 
2 
2 
PEPD-2* 4 ’ 5 
M 
2 
3 
Superoxide dismutase 
1.15.1.1 
sSOD- 1 * 4 - 5 
L 
7 
1 
mSOD* 
H 
1,2 
1 
Triose-phosphate isomerase 
5.3.1. 1 
TPI-P" 
E 
7 
1 
TPI-2* 4 
E 
7 
2 
TPI-3* 4 
E 
7 
1 
TPl-4 * 4 
E 
7 
2 
1 E = eye; H = heart; L = liver; M = muscle; preferred tissue listed first. 
2 1 = lithium hydroxide (Ridgway et al., 1970); 
2 = Tris-EDTA-borate (Boyer et al., 1963); 
3 = amine citrate, pH 6.1 (Clayton and Tretiak, 1972); 
4 = Tris-citrate, pH 7.0 (Shaw and Prasad, 1970); 
5 = Tris-citrate discontinuous (Schaal and Anderson, 1974); 
6 = amine-citrate-EDTA, pH 7.2 (modified from Clayton and Tretiak, 1972); 
7 = Tris-glycine (Holmes and Masters, 1970); 
8 = amine-citrate, pH 6.8 (modified from Clayton and Tretiak, 1972). 
3 1 = monomorphic; 
2 = low (frequency of most prevalent allele >0.95); 
3 = high (frequency of most prevalent allele <0.95). 
4 Loci included in 36-locus version of neighbor-joining tree and gene diversity analysis. 
5 Loci included in 21-locus version of neighbor-joining tree. 
are made for multiple testing. This is especially notable 
for the series of collections from the Znamenka (9 collec- 
tions, P=0.43) and Ochepukha (6 collections, P=0.52) riv- 
ers. No heterogeneity was detected among Sakhalin drain- 
ages (G= 131.01, 127 df; P=0.39). 
The three Japanese (Hokkaido) collection sites were 
the Tokushibetsu River on the Sea of Okhotsk coast, the 
Kushiro River facing the northwestern Pacific Ocean, and 
the Yurappu River southwest of the Kushiro River on Hok- 
kaido’s eastern coast. The synthetic Yurappu stock is de- 
rived in large part from Okhotsk coast stocks, including 
the Tokushibetsu stock. The genetic profile of the Yurap- 
pu stock resembles that of the Tokushibetsu stock except 
at sAAT-3* (P=0.018, analysis not shown), which suggests 
a founder effect or subsequent divergence. The Yurappu 
sample was not used in subsequent analyses. Overall het- 
erogeneity was observed between the Kushiro and Tokush- 
ibetsu Rivers (G=48.87, 33 df;P=0.037) (Table 2), although 
tests at the four loci suggest heterogeneity was not signifi- 
cant after correction for multiple testing. 
