186 BULLETIN OF THE BUREAU OF FISHERIES 
Table 14. — The effect of soluble fraction of crude oil from Lake Barre and Lake Pelto wells on the rate 
of 'pumping of water by the gills of the oyster 
[Detailed record of the experiments. Figures printed in heavy type represent observations made when oysters are in solution of 
soluble fraction] 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
11 
12 
13 
14 
15 
16 
17 
18 
Time 
Experiment 
Date 
Crude oil 
Of 
treat- 
Drops per minute, average by 5-minute intervals 
ment 
5 
10 
15 
20 
25 
30 
35 
40 
45 
50 
55 
60 
65 
70 
75 
80 
85 
90 
1933 
Min. 
60 
Nov. 28 
Barre 
5 
94 
92 
91 
87 
S3 
110 
118 
101 
104 
98 
91 
88 
60A 
__ do._- 
_ _ do 
5 
61 
64 
64 
65 
75 
29 
37 
58 
61 
69 
73 
76 
62 
Dee. 1 
_do __ 
5 
34 
35 
31 
30 
24 
13 
18 
26 
29 
27 
25 
63 A 
Dec. 4 
do 
5 
115 
114 
113 
118 
101 
85 
90 
101 
120 
106 
100 
107 
64A 
Dee. 5 
do 
5 
80 
81 
74 
75 
76 
14 
14 
62 
75 
75 
68 
68 
65 A 
Dec. 7 
Pelto 
5 
44 
42 
40 
39 
42 
42 
51 
47 
44 
40 
34 
38 
66 
Dec. 11 
do 
10 
35 
35 
40 
34 
30 
22 
27 
52 
45 
38 
38 
37 
67 
__ do 
do 
10 
63 
59 
64 
56 
57 
52 
57 
61 
62 
54 
56 
68 
Dee. 12 
do _ 
10 
70 
72 
59 
68 
81 
31 
44 
52 
51 
54 
56 
68 
69 
Dec. 15 
do 
13 
65 
81 
80 
82 
81 
73 
40 
35 
73 
84 
83 
72 
70 
Dec. 21 
do 
15 
119 
122 
121 
121 
1 
1 
1 
0 
0 
29 
42 
39 
52 
70A 
Dec. 22 
Sea water . 
15 
80 
83 
85 
80 
2 
2 
2 
25 
25 
28 
75 
91 
91 
73 . 
1934 
May 9 
May 10 
May 16 
Control 
46 
49 
46 
36 
45 
42 
42 
43 
40 
36 
35 
32 
42 
38 
41 
39 
74 
do __ 
127 
128 
118 
126 
106 
127 
126 
120 
134 
117 
142 
144 
116 
141 
126 
144 
84 
do 
141 
136 
144 
140 
141 
138 
136 
143 
143 
141 
136 
140 
144 
139 
139 
131 
136 
135 
86 _ 
do 
_ __do 
123 
122 
104 
113 
117 
117 
109 
112 
113 
114 
116 
117 
71 
May 7 
May 9 
May 10 
do... 
Barre 
5 
118 
115 
in 
107 
104 
103 
49 
40 
58 
63 
64 
67 
71 
78 
70 
77 
72 
do 
5 
125 
116 
133 
137 
74 
32 
88 
91 
100 
108 
111 
108 
118 
107 
112 
93 
75 __ 
do 
10 
122 
54 
45 
55 
54 
45 
43 
47 
52 
56 
48 
44 
44 
56 
65 
69 
107 
76 
do 
12 
124 
129 
100 
71 
88 
104 
114 
105 
136 
114 
138 
140 
77 
___do 
do 
10 
123 
12.5 
72 
64 
SO 
76 
83 
67 
80 
94 
66 
100 
107 
110 
120 
110 
118 
78 
May 14 
___do 
do 
10 
148 
136 
77 
149 
123 
53 
0 
0 
0 
119 
220 
131 
136 
129 
114 
140 
133 
137 
79 
- ___do 
10 
146 
0 
0 
0 
0 
42 
130 
139 
133 
149 
148 
80 
_ do 
do 
10 
153 
103 
4 
0 
0 
0 
6 
78 
109 
105 
133 
139 
147 
143 
144 
149 
81 .. 
May 15 
___do 
__do . 
8 
151 
161 
156 
155 
101 
G7 
84 
125 
130 
135 
129 
125 
134 
134 
133 
131 
82 
do _ 
8 
133 
131 
135 
132 
61 
44 
117 
120 
121 
117 
117 
120 
83.. 
--_do 
do 
20 
120 
127 
126 
130 
80 
27 
30 
21 
i 
108 
117 
102 
89 
100 
98 
95 
97 
85 
May 16 
May 17 
May 21 
May 22 
do 
24 
137 
133 
129 
134 
104 
39 
31 
12 
0 
0 
71 
102 
105 
99 
104 
112 
119 
87 
do 
24 
87 
87 
87 
85 
64 
45 
32 
21 
1 
2 
37 
52 
54 
55 
56 
45 
60 
88 
_do 
38 
151 
155 
154 
156 
120 
69 
54 
52 
49 
34 
2 
0 
0 
29 
82 
107 
108 
89. __ 
do 
12 
118 
130 
131 
128 
127 
127 
121 
125 
85 
15 
16 
37 
84 
86 
84 
90 
95 
The effect of temperature on the rapidity of recovery is equally striking. The 
oysters in both winter and spring experiments show, after treatment with soluble 
fraction solution, a fairly steady rise in the rate of pumping but this initial rise is 
followed by a slight drop (fig. 9). The significance, if any, of this peak in the recovery 
curve is not known. However, it is useful in demonstrating the relation of temper- 
ature to rate and extent of recovery. In table 12, the column headed “Recovery 
time”, shows the percent of recovery at the peak as compared with the average rate 
before treatment. The time in minutes extends from the moment of turning off 
the test solution to the point where the peak occurs. For the winter series, the 
average recovery at the peak was 109 percent in an average time of 13.5 minutes. 
The spring series averaged only 78 percent recovery at the peak in an average time 
of 21.6 minutes (table 13). 
Results of the experiment carried out at Woods Hole, Mass., in which a solution 
of water-soluble substances present in Pelto oil was prepared by allowing them to 
diffuse through a collodion sack suspended in the sea water are similar to those ob- 
tained at Beaufort with soluble fraction prepared by shaking oil with the sea water. 
The sack, about 1 inch in diameter and 7 inches high, was made by dipping a glass 
test tube of corresponding size into a solution of Merck’s collodion (reagent quality) 
and drying it for 1 minute. The membrane was carefully examined under a micro- 
scope and tested against leakage. Fifty cubic centimeters of oil were poured into 
the sack and the latter was kept suspended for 4 days in 4 liters of sea water which 
was gently agitated by means of an electric stirrer with glass rod. The specific 
